Silver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines

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dc.contributor.authorDarvish, Soodabeh
dc.contributor.authorKahrizi, Mohammad Saeed
dc.contributor.authorÖzbolat, Gülüzar
dc.contributor.authorKhaleghi, Fatemeh
dc.contributor.authorMortezania, Zohreh
dc.contributor.authorSakhaei, Delaram
dc.date.accessioned2025-03-23T19:17:03Z
dc.date.available2025-03-23T19:17:03Z
dc.date.issued2022
dc.departmentSinop Üniversitesi
dc.description.abstractObjective(s): A study was performed on the possibility of synthesizing silver nanoparticles (Ag-NPs) from Ducrosia Anethifolia Aqueous Extract for treating breast cancer MCF-7 and MDA-MB-231 cell lines. Methods: The application of Ducrosia Anethifolia Aqueous Extract caused a reduction in silver ions and facilitated the production of silver nanoparticles. Different analyzing procedures were exerted to evaluate the qualities of synthesized NPs by Ducrosia Anethifolia Aqueous Extract including UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). In the following, subsequent to assessing the viability of MCF- 7 and MDA-MB-231 cell lines, their exposure to AgNPs was evaluated by the usage of MTT assay. Moreover, the Bax, Bad, Bcl-2, and c-FLIP expression levels of treated cells were examined by real-time PCR analysis. Results: The observance of an absorption peak at 460 nm through the obtained data from a spectrophotometer affirmed the production of silver NPs. The images of electron microscope displayed the spherical shape of NPs in an average size of around 9.41 nm. Moreover, this product caused a robust reduction in the viability of both MCF-7 and MDA-MB-231 cell lines that was mainly pointed out by the improved expressions of Bax and Bad gene, while minimizing the gene expressions of Bcl-2 and c-FLIP. Conclusions: This experiment approved the applicability of Ducrosia Anethifolia Aqueous Extract for performing the green synthesis of NPs. In addition, the synthesized NPs exhibited anti-tumor effects on the applied breast cancer cell lines through the up-regulation of pro-apoptotic proteins concomitant along with the down-regulation of anti-apoptotic proteins expression. © Semina:Ciencias Agrarias.All right reserved.
dc.identifier.doi10.22034/nmrj.2022.01.008
dc.identifier.endpage92
dc.identifier.issn2476-3489
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85132328104
dc.identifier.scopusqualityQ3
dc.identifier.startpage83
dc.identifier.urihttps://doi.org/10.22034/nmrj.2022.01.008
dc.identifier.urihttps://hdl.handle.net/11486/4257
dc.identifier.volume7
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTehran University of Medical Sciences
dc.relation.ispartofNanomedicine Research Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250323
dc.subjectbiosynthesis
dc.subjectbreast cancer
dc.subjectDucrosia Anethifolia
dc.subjectSilver nanoparticle
dc.subjectviability
dc.titleSilver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines
dc.typeArticle

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