Characterization of Environmental Bacillus Isolates by Protein Fingerprinting and Random Amplified Polymorphic DNA Profiles

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dc.contributor.authorBerber, Ismet
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorYegin, Zeynep
dc.contributor.authorCivek, Seyhan
dc.date.accessioned2025-03-23T19:16:33Z
dc.date.available2025-03-23T19:16:33Z
dc.date.issued2017
dc.departmentSinop Üniversitesi
dc.description.abstractThe aim of the present study was to investigate antibiotic resistance, plasmid, random amplified polymorphic DNA (RAPD) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles of the Bacillus spp. isolated from soil of Sinop environs, Turkey. A total of fifty-seven isolates were identified as Bacillus spp. based on morphological and physiological properties. All isolates were found resistant to bacitracin. Susceptibility patterns of the isolates to other tested antibiotics varied from 100 to 31.5 %. The plasmid analysis results indicated that isolates harbored 2 to 7 plasmids. The numerical analysis of whole cell and extracellular protein profiles of the isolates displayed similarity levels between 78–100 and 67–100 % respectively reflecting the less discriminative power of SDS-PAGE at subspecies level. However, RAPD analysis of genomic DNA using four different universal primers revealed 100 % polymorphism indicating the strength of RAPD in the differentiation of Bacillus isolates. © 2016, The National Academy of Sciences, India.
dc.identifier.doi10.1007/s40011-015-0700-0
dc.identifier.endpage1259
dc.identifier.issn0369-8211
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85032352704
dc.identifier.scopusqualityQ2
dc.identifier.startpage1247
dc.identifier.urihttps://doi.org/10.1007/s40011-015-0700-0
dc.identifier.urihttps://hdl.handle.net/11486/4125
dc.identifier.volume87
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer India
dc.relation.ispartofProceedings of the National Academy of Sciences India Section B - Biological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250323
dc.subjectAntibiotic resistance
dc.subjectBacillus
dc.subjectMolecular characterization
dc.subjectProtein fingerprinting
dc.subjectRandom amplified polymorphic DNA
dc.titleCharacterization of Environmental Bacillus Isolates by Protein Fingerprinting and Random Amplified Polymorphic DNA Profiles
dc.typeArticle

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