Identification and Protein Fingerprinting of Staphylococcus aureus Species Isolated from Tooth Decay

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dc.contributor.authorBilgin Çine, Nevra
dc.contributor.authorBerber, İsmet
dc.contributor.authorAvşar, Cumhur
dc.date.accessioned2025-03-23T19:16:33Z
dc.date.available2025-03-23T19:16:33Z
dc.date.issued2017
dc.departmentSinop Üniversitesi
dc.description.abstractStaphylococcusaureus is the most prevalent human pathogenic microorganism worldwide. In the present study, a total of 57 S. aureus strains, including 54 clinical isolates obtained from dental hospitals and three reference strains were characterized by phenotypic characteristics and SDS-PAGE protein profiling. Out of the 132 clinical specimens, 54 S. aureus strains were isolated and identified according to their morphological and physiological characteristics. The strains were tested against 13 standard antibiotics by using disc diffusion procedure. Antibiogram results showed that the highest resistance among the isolates was determined against ampicillin (83.6 %), whereas they were susceptible to sulfamethoxazole/trimethoprim, cefoperazone/sulbactam, vancomycin and imipenem. In addition, multidrug resistance index values of 12 (22.2 %) of the isolates were higher than the reference value of 0.2. The numerical analysis of whole-cell protein profiles of S. aureus strains revealed 2 major clusters at similarity levels of 57 % or above. Furthermore, the numerical analysis of extra-cellular protein profiles of S. aureus strains yielded 2 major clusters at similarity levels of 48 % or above. In conclusion, the present findings revealed that use of conventional methods with the numerical analysis of whole-cell and extra-cellular protein profiles might be useful for the more reliable identification of S. aureus strains isolated from tooth decay. © 2015, The National Academy of Sciences, India.
dc.identifier.doi10.1007/s40011-015-0569-y
dc.identifier.endpage22
dc.identifier.issn0369-8211
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85014866597
dc.identifier.scopusqualityQ2
dc.identifier.startpage13
dc.identifier.urihttps://doi.org/10.1007/s40011-015-0569-y
dc.identifier.urihttps://hdl.handle.net/11486/4124
dc.identifier.volume87
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer India
dc.relation.ispartofProceedings of the National Academy of Sciences India Section B - Biological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250323
dc.subjectAntibiogram
dc.subjectNumerical analysis
dc.subjectS. aureus
dc.subjectSDS-PAGE
dc.subjectTooth decay
dc.titleIdentification and Protein Fingerprinting of Staphylococcus aureus Species Isolated from Tooth Decay
dc.typeArticle

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