Ulva spp. (Sinop, Karadeniz) Türünün Yağ Asitleri, ? Tokoferol ve Toplam Pigment Miktarının Araştırılması
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Dosyalar
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Durmaz, Y., Duyar, H. A., Gökpınar, Ş., Öğretmen, Y. Ö., Bandarra, N. M., "Ulva spp. (Sinop, Karadeniz) Türünün Yağ Asitleri, ? Tokoferol ve Toplam Pigment Miktarının Araştırılması". Journal of Fisheriesscience.com.
Erişim Hakkı
Özet
Deniz yosunlarıyüksek miktarda protein, yağ asitleri ve mineral maddeler içerir. Makro-alglerin biyokimyasal içerikleri tür, ışık, sıcaklık v.b. koşullara bağlı olarak değişir. Tuzluluk ve sıcaklık koşullarına bağlı olarak Karadeniz, makro-algler bakımından yüksek tür çeşitliliğine sahiptir. Bu çalışmada Karadeniz'in Sinop kıyılarından toplanan Ulva spp. 60°C etüvde kurutuldu, toz haline getirildi ve analizler yapılıncaya kadar 20°C'de muhafaza edildi. Örneklerdeki pigmentler (klorofila ve toplam karotenoyit) spektrofotometrik yöntem ile analiz edildi, yağ asitleri Varian GC kullanılarak belirlendi ve ?-tokoferol tespiti için Jasco HPLC kullanıldı. Örneklerde toplam doymuş yağ asitleri (SAT) % 37.2, toplam çoklu doymamış yağ asitleri (PUFA) %32.0, toplam tekli doymamış yağ asitleri (MUFA) % 23,2 olarak tayin edildi. PUFA grubundan 18:3(n-3) % 5.4, 18:3(n-6) % 1.6, 18:4 (n-3) % 6.5 ve 20:5 (n-3) % 4.4 gibi yağ asitlerinin yüksek değerlerde olduğu tespit edildi. Ulva spp.'de ?-tokoferol değeri 9.1 ±0.5 ?gg-1 kuru ağırlık (dw) olarak tespit edildi. Toplam karoten ve toplam klorofil a düzeyleri ise sırasıyla 311.0 ±0.3 ?g g -1 dw ve 706.8 ±0.7 ?g g -1 dw olarak saptandı. Makro-alg türleri ve bunlar arasında Ulva spp. Türk sularında en yaygın dağılıma sahip ve yüksek biyomaslara ulaşan bir Chlorophyceae üyesidir. Yüksek düzeyde pigment, yağ asidi ve vitamin değerlerine sahip olan bu türün olası tüketim yollarının aranması, Türk sularının bu değerli biyolojik kaynağının çok daha etkin bir şekilde kullanılmasını sağlayacaktır.
Marine algae contain large amounts of protein, fatty acids and minerals. Biochemical compo- sition of macro algae depends on the species, on conditions like light, temperature and so on. Black Sea has a high variety of species with re spect to macro-algae because of salinity and temperature conditions. In this study, Ulva spp. which was collected from Black Sea on the coast of Sinop was dried in an oven at 60°C, grinded as a powder and kept at 20°C until ana- lyses were carried out. Pigments (chlorophyl a and total carotenoid) of samples were analysed with spectrophotometric method, fatty acids were analysed using Varian GC, and HPLC was used for the estimation of ? - tocopherol. Total saturated fatty acids (SAT) were determined to be 37.2 %, total polyunsaturated fatty acids (PUF A) to be 32.0 %, and total monounsaturated fatty acids (MUFA) were determined to be 23.2 %. Within the PUFA`s, fatty acids such as; 18:3(n-3) 5.4 %, 18:3(n-6) 1.6 %, 18:4(n-3) 6.5 % and 20:5(n-3) 4.7 % exhibited high values. ? -tocopherol value for Ulva spp. was measured as 9,1 ±0,5 ?g g -1 dw. Total carotenoid and to- tal chlorophyl a levels were measured as 311.0± 0.3 ?g g -1 dw and 706.8 ±0.7 ?g g -1 dw, respectively. Macro-algae species, and among them Ulva spp., are a member of Chlorophyceae which have the highest distributi on in Turkish waters and which attain high biomass. Research into possible ways of use for this species which has high levels of fatty acid, vitamin and pigment will bring about more effective use of this biological source of Turkish waters
Marine algae contain large amounts of protein, fatty acids and minerals. Biochemical compo- sition of macro algae depends on the species, on conditions like light, temperature and so on. Black Sea has a high variety of species with re spect to macro-algae because of salinity and temperature conditions. In this study, Ulva spp. which was collected from Black Sea on the coast of Sinop was dried in an oven at 60°C, grinded as a powder and kept at 20°C until ana- lyses were carried out. Pigments (chlorophyl a and total carotenoid) of samples were analysed with spectrophotometric method, fatty acids were analysed using Varian GC, and HPLC was used for the estimation of ? - tocopherol. Total saturated fatty acids (SAT) were determined to be 37.2 %, total polyunsaturated fatty acids (PUF A) to be 32.0 %, and total monounsaturated fatty acids (MUFA) were determined to be 23.2 %. Within the PUFA`s, fatty acids such as; 18:3(n-3) 5.4 %, 18:3(n-6) 1.6 %, 18:4(n-3) 6.5 % and 20:5(n-3) 4.7 % exhibited high values. ? -tocopherol value for Ulva spp. was measured as 9,1 ±0,5 ?g g -1 dw. Total carotenoid and to- tal chlorophyl a levels were measured as 311.0± 0.3 ?g g -1 dw and 706.8 ±0.7 ?g g -1 dw, respectively. Macro-algae species, and among them Ulva spp., are a member of Chlorophyceae which have the highest distributi on in Turkish waters and which attain high biomass. Research into possible ways of use for this species which has high levels of fatty acid, vitamin and pigment will bring about more effective use of this biological source of Turkish waters
Açıklama
Anahtar Kelimeler
Ulva spp, ? -tokoferol, Pigment, Yağ asidi, ? -tocopherol, Fatty acids
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Journal of Fisheries Science.com. 2(3), 350-356 (2008).
