Numerical Analysis of SDS-PAGE Protein Patterns of Facultative Alkaliphilic Bacillus Species Isolated From Lake Van, Turkey

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dc.contributor.authorBerber, İsmet
dc.contributor.authorBerber, Şerife
dc.date.accessioned2014-09-03T13:26:27Z
dc.date.available2014-09-03T13:26:27Z
dc.date.issued2006
dc.description.abstractIn the present study, seven reference Bacillus species and a total of eighteen new facultative alkaliphilic Bacil-lus strains, isolated from the water of Lake Van and its soil surroundings, were identified using phenotypic charac-teristics and the numerical analysis of whole-cell protein profiles. According to morphological, biochemical and physiological characteristics, it was found that the new isolated strains belonged to Bacillus genus. In addition, the research indicated that SDS-PAGE of polypeptides of whole-cell extracts can provide more valuable taxonomic information than conventional tests based on the pheno-typic characteristics at the species and subspecies levels. Numerical analysis of whole-cell protein profiles of test strains revealed 4 basic clusters (I-IV) at dissimilarity val-ues of 18.9 % or above. The results of numerical analysis confirmed that each cluster had characteristic and distinc-tive protein profiles. Indeed, this study showed that the application of numerical analysis, coupled with the utiliza-tion of a standardized identification system instead of sim-ple quantitative comparison of protein patterns, greatly enhanced the utilization of whole-cell protein profiles for identification of the facultative alkaliphilic Bacillus species.
dc.identifier.citationBerber, İ., Berber, Ş. "Numerical Analysis of SDS-PAGE Protein Patterns of Facultative Alkaliphilic Bacillus Species Isolated From Lake Van, Turkey", Fresenius Environmental Bulletin, Vol.15, No.5, pp.409-416.
dc.identifier.urihttp://www.psp-parlar.de/pdf/F_25_222_OriginalPaper_pp409_416.pdf
dc.identifier.urihttps://hdl.handle.net/11486/526
dc.language.isoen
dc.publisherFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Kategorisiz
dc.subjectLake Van
dc.subjectBacillus spp.
dc.subjectNumerical Analysis
dc.subjectProtein profiles
dc.subjectPhenotypic characterization
dc.subjectSDS-PAGE
dc.titleNumerical Analysis of SDS-PAGE Protein Patterns of Facultative Alkaliphilic Bacillus Species Isolated From Lake Van, Turkey
dc.typeArticle

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