Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya-Terziköy Thermal Spring

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dc.contributor.authorTatar, Demet
dc.contributor.authorVeysioglu, Aysel
dc.contributor.authorTokatli, Ali
dc.contributor.authorDuyar, Hunkar Avni
dc.date.accessioned2026-04-25T14:20:35Z
dc.date.available2026-04-25T14:20:35Z
dc.date.issued2025
dc.departmentSinop Üniversitesi
dc.description.abstractActinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terzik & ouml;y Thermal Spring in Amasya, T & uuml;rkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates-specifically amylase and protease production-were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlightthe phylogenetic diversity ofthermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.
dc.identifier.endpage284
dc.identifier.issn2602-473X
dc.identifier.issue4
dc.identifier.orcid0000-0002-7559-8882
dc.identifier.scopusqualityQ3
dc.identifier.startpage278
dc.identifier.urihttps://hdl.handle.net/11486/8652
dc.identifier.volume40
dc.identifier.wosWOS:001594193900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherIstanbul Univ Press, Fac Aquatic Sciences
dc.relation.ispartofAquatic Sciences and Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260420
dc.subjectThermophilic bacteria
dc.subject16S rRNA gene
dc.subjectamylase and protease activities
dc.titleDetermination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya-Terziköy Thermal Spring
dc.typeArticle

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