Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya- Terziköy Thermal Spring

Basit Öğe Kaydı
dc.contributor.authorTatar, Demet
dc.contributor.authorDuyar, Hünkar Avni
dc.contributor.authorVeyisoglu, Aysel
dc.contributor.authorYılmaz, Adem Barış
dc.date.accessioned2026-04-25T14:11:32Z
dc.date.available2026-04-25T14:11:32Z
dc.date.issued2025
dc.departmentSinop Üniversitesi
dc.description.abstractActinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terziköy Thermal Spring in Amasya, Türkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates—specifically amylase and protease production—were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlight the phylogenetic diversity of thermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.
dc.identifier.doi10.26650/ASE.2025.1757673
dc.identifier.endpage284
dc.identifier.issn2602-473X
dc.identifier.issue4
dc.identifier.startpage278
dc.identifier.trdizinid1355725
dc.identifier.urihttps://doi.org/10.26650/ASE.2025.1757673
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1355725
dc.identifier.urihttps://hdl.handle.net/11486/7936
dc.identifier.volume40
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAQUATIC SCIENCES and ENGINEERING
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR_20260420
dc.subjectBiyoloji
dc.subjectMikrobiyoloji
dc.titleDetermination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya- Terziköy Thermal Spring
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu