Sinop’da Yetişen Bazı Bitkilerin Metanolik Ekstraktlarının Antibakteriyal ve Antifungal Aktivitelerinin Belirlenmesi
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Date
2013
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Karaelmas Science and Engineering Journal
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Abstract
Bu çalışmada, Sinop’da yetişen 15 farklı bitki türünden elde edilen metanol ekstraktların antimikrobiyal aktiviteleri 3 Gram-pozitif (Staphylococcus aureus ATCC 6538, Micrococcus luteus ATCC 9345, Bacillus cereus ATCC 7064), 2 Gram-negatif (Escherichia coli ATCC 11293 ve Enterococcus faecalis ATCC 51299) ve 3 maya (Candida krusei ATCC 6258, C. parapilosis ATCC 22019 ve C. albicans ATCC 14053) suşlarına karşı disk difüzyon yöntemi kullanılarak test edildi. Antimikrobiyal etkisi incelenen bitki türlerinin tümünün bir veya daha fazla mikroorganizmaya karşı etkili olduğu bulundu. Ayrıca, Sinop’a endemik olan Crocus speciosus subsp. xantholaimos’ın test edilen Gram-pozitif ve Gram-negatif bakterilere karşı yüksek antibakteriyal aktiviteye sahip olduğu belirlendi. Buna karşın, Laurus nobilis yaprak ve meyve özütleri 3 Candida türüne karşı daha yüksek antifungal aktivite gösterdi. Genel olarak bu çalışmadan elde edilen sonuçlar, incelen bitki ekstraktlarının antibakteriyal ve antifungal aktiviteleri arasında önemli bir farkın olmadığını gösterdi.
In the present study, the antimicrobial activities of 15 different plant species growing in Sinop were tested against 3 Gram-positive (Staphylococcus aureus ATCC 6538, Micrococcus luteus ATCC 9345, Bacillus cereus ATCC 7064), 2 Gram-negative (Escherichia coli ATCC 11293 and Enterococcus faecalis ATCC 51299) and 3 yeast (Candida krusei ATCC 6258, C. parapilosis ATCC 22019 and C. albicans ATCC 14053) strains by using disc diffusion method. It was found that all examined plant species in point of the antimicrobial activity was effective against one or more species of microorganisms. Additionally, it was determined that Crocus speciosus subsp. xantholaimos endemic to Sinop had high antimicrobial activity against tested Gram-positive and Gram-negative bacteria. However, the leaf and fruit extracts of Laurus nobilis displayed higher antifungal activity against 3 Candida species. In general, the results of the study showed that there was no significant difference between antibacterial and antifungal activities of the investigated plant extracts.
In the present study, the antimicrobial activities of 15 different plant species growing in Sinop were tested against 3 Gram-positive (Staphylococcus aureus ATCC 6538, Micrococcus luteus ATCC 9345, Bacillus cereus ATCC 7064), 2 Gram-negative (Escherichia coli ATCC 11293 and Enterococcus faecalis ATCC 51299) and 3 yeast (Candida krusei ATCC 6258, C. parapilosis ATCC 22019 and C. albicans ATCC 14053) strains by using disc diffusion method. It was found that all examined plant species in point of the antimicrobial activity was effective against one or more species of microorganisms. Additionally, it was determined that Crocus speciosus subsp. xantholaimos endemic to Sinop had high antimicrobial activity against tested Gram-positive and Gram-negative bacteria. However, the leaf and fruit extracts of Laurus nobilis displayed higher antifungal activity against 3 Candida species. In general, the results of the study showed that there was no significant difference between antibacterial and antifungal activities of the investigated plant extracts.
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Antimikrobiyal etki, Disk difüzyon yöntemi, Endemik, C. speciosus subsp. xantholaimos, Antimicrobial activity, Disc diffusion method, Endemic
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Berber, İ., Avşar, C., Çine, N., Bzokurt, N., Elmas, E. "Sinop’da Yetişen Bazı Bitkilerin Metanolik Ekstraktlarının Antibakteriyal ve Antifungal Aktivitelerinin Belirlenmesi". Karaelmas Science and Engineering Journal, 3(1):10-16.
