The Influence of Pesticides on the Spore Viability, Toxin Stability and Larvicidal Activity of Bacillus sphaericus 2362 Strain
Yükleniyor...
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Fresenius Environmental Bulletin
Erişim Hakkı
Özet
In this study, 12 different commercial pesticides, in-cluding 5 fungicides, 5 herbicides, 1 aphicide and 1 acari-cide, were investigated for their effects on spore viability, toxin stability and larvicidal activity of a mosquito patho-genic B. sphaericus 2362 strain. Minimal inhibitory con-centrations (MICs) of each pesticide were determined after 48 h incubation of B. sphaericus 2362 strain. Ac-cording to MIC results, all pesticides showed various inhibitory effects on the growth of the test bacterium. Particularly, the pesticides which contain TCA, hymexa-zol, copper sulphate, hexythiazox, 2,4-D Amine, copper inhibited spore germination significantly and bacterial growth even at the lowest tested concentrations. Our re-sults indicated that various commercial pesticides in dif-ferent concentrations reduced the spore viability signifi-cantly and heat-resistant spore numbers of B. sphaericus 2362 strain. In general, the pesticides decreased the spore numbers 104-105-fold compared to initial ones. Further-more, it was observed that the larvicidal activity was completely lost when it was grown at the concentration of MIC values or higher. Indeed, SDS-PAGE analysis con-firmed that the binary toxin proteins, 41.9 kDa and 51.4 kDa, were lost at the MIC values and higher concentra-tions although parasporal crystals did not disappear in the much lower pesticide concentration.
Açıklama
Anahtar Kelimeler
Pesticides, B. sphaericus 2362, Toxin stability, Larvicidal activity and SDS-PAGE
Kaynak
WoS Q Değeri
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Sayı
Künye
Berber, İ., Atalan, E., Çökmüş, C. "The Influence of Pesticides on the Spore Viability, Toxin Stability and Larvicidal Activity of Bacillus sphaericus 2362 Strain", Fresenius Environmental Bulletin, Vol.13, No: 5, pp.424-429.
