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    DEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS Puccinia triticina
    (Serbian Genetics Soc, 2015) Sipahi, Huelya; Yumurtaci, Aysen; Mert, Zafer
    This study focused on the development of EST-simple sequence repeats markers and the detection of their transferability and their utility for evaluating wheat leaf rust pathogen diversity. A total of 44,407 publicly available EST sequences derived from Puccinia triticina were computationally mined. Di-nucleotide repeat density covered the vast majority of assembled ESTs (45%). The tri-repeat motif (TCT) and penta-repeat motif (TTCTT) were the most repeated motif. A set of 103 Class I type sequences containing simple sequence repeats were further analyzed by BLASTX similarity. Nineteen primer pairs flanking regions of EST-SSRs were designed. Of the 19 primer pairs tested, 10 successfully amplified fragments. Their polymorphism was evaluated with 8 Puccinia triticina (Pt) single-uredinal isolates collected from the different regions of Turkey. These newly developed EST-SSR primer pairs can be implicated as stable markers for pathogen diversity analysis. It was also shown that some leaf rust EST-SSR markers were capable of cross-amplification in P. graminis f. sp. tritici.
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    Öğe
    Volatile Profiles, Antioxidant, and Antimicrobial Activities of Essential Oils and Extracts of Different Parts from Sarcopoterium spinosum (L.) Spach Growing Wild in Sinop (Turkey)
    (Taylor & Francis Ltd, 2017) Sipahi, Huelya; Korkmaz, Buesra; Avsar, Cumhur; Fandakli, Seda; Elmas, Emire
    Volatile compositions of the hydrodistillated essential oils (EOs), SPME and SPME of hexane extracts from stems, leaves and roots of Sarcopoterium spinosum (L.) Spach. were investigated by means of GC and GC-MS. Vegetative organs were found to contain different volatile compounds. Oxygenated monoterpenes (20.7 %), aromatic compounds (33.0 %) and aldehydes (39.6 %) were identified as the main components in the EO of stems. Oxygenated sesquiterpenes (25.7 %), aliphatic hydrocarbons (21.2 %) and aldehydes (42.5 %) were found in high percentages in the EO of leaves, while aromatic compounds (48.4 %) and aldehydes (51.6 %) were found to be major constituent in the EO of roots. Aldehydes were the major groups in SPME of stems, leaves, and roots (81.3.2 %, 54.5 % and 41.7 %, respectively). The volatiles of the SPME analysis of hexane extracts were distinguished by predomination of aromatic compounds (68.0 % for stems and 73.1 % for leaves) and ketones (27.5 % for stems and 22.3 % for leaves). Hexane extract of leaves had antimicrobial property against Staphylococcus aureus and Bacillus cereus. But, hexane extract of stems only displayed activity against S. aureus. The methanol extracts of stems and leaves exhibited high activity against Methicillin Resistant S. aureus (MRSA), S. aureus, and Micrococcusluteus. All EOs inhibited the growth of Candida krusei, C. parapsilosis, MRSA, Pseudomonas aeruginosa, S. aureus, Vancomycin Resistant Enterococcus (VRE) and B. cereus. Furthermore, antioxidant activity of hexane extracts of stem and leaves and EOs of leaves were higher than that of methanol extracts.