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    Transferability of barley and wheat EST-microsatellite markers in some Poaceae members
    (Sinop Üniversitesi, 2016) Sipahi, HÜLYA; Aslan, Yeliz; Yumurtaci, Aysen
    The cross species transferability of  barley and wheat microsatellite markersdeveloped from expressed sequence tag (EST) libraries constructed under Fusarium infection conditions were detected among 17 species including 8from Aegilops, 6 from Triticum, Zea mays, Avena sativa, Oryzasativa.Transferability rates of barleymicrosatellite primer pairs ranged from 29% to 100%. A maximum of 100%cross-genera transferability noticed with Avena followed by Zea (92%),Triticum (83%), Aegilops (68%), and Oryza (8%). Primerpairs were highly transferable within species of Triticum (100% in T.turgidum durum durum, 92% in T. turgidum durum dicoccon and T.monococcum aegilopoides, 83% in T.timopheevii timopheevii and T.turgidum dicoccoides, 67% in T. timopheevii armeniacum). Only oneprimer pairs (contig624) showed100 % cross-species/genera amplification in all materials studied. Considering wheat microsatellites, the microsatelliteprimer pairs were highly transferable within species of Triticum (rangedfrom %100 to %70) and but low transferable in the allied cereals (15% inAvena, 50% in Oryza, 45% in Zea, 60% in Hordeum). Twoprimer pairs have shown transferability only in some Triticum species,while two others showed amplication only in species of Aegilops and Triticum.Only one primer pairs showed 100 % cross-species/genera amplification in allmaterials studied.This highercross-species transferability of EST microsatellite markers indicated a highlevel of conservation of DNA sequences belonging to the transcribed region ofthe genome and its suitability in comparative genome mapping, genetic diversityand phylogenetics analysis.