Yazar "Yilmaz, Muhitdin" seçeneğine göre listele

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    EFFECTS OF GLYPHOSATE (HERBICIDE) ON SERUM PARAOXONASE ACTIVITY, HIGH DENSITY LIPOPROTEIN, TOTAL ANTIOXIDANT AND OXIDANT LEVELS IN KARS CREEK TRANSCAUCASIAN BARBS (CAPOETA CAPOETA [GULDENSTAEDT, 1773])
    (Parlar Scientific Publications (P S P), 2017) Deveci, Haci Ahmet; Unal, Sevgi; Karapehlivan, Mahmut; Ayata, Muradiye Karasu; Gaffaroglu, Muhammet; Kaya, Inan; Yilmaz, Muhitdin
    The aim of this study is to explore the effects of the herbicide glyphosate on serum paraoxonase (PON1) activity, high-density lipoprotein (HDL), and total antioxidant (TAS) and oxidant (TOS) levels in Kars Creek Transcaucasian Barbs (Capoeta capoeta [Guldenstaedt, 1773]). Twenty four Capoeta capoeta are chosen within a weight range of 200 to 240 grams and they are equally divided into 3 groups, namely Groups I, II, III. The fish placed in Group I are received aquatic environment while Groups II and III were kept in aquatic environments with a pH value of 7.4 in tanks to which 0,01 and 0,02 mg/L glyphosate (N-phosphonomethyl glycine 480 mg/L), respectively. It was found that groups II and III had significantly lower serum plasma PON1, HDL and TAS levels than the control group (Group I). On the other hand, serum TOS levels were found significantly elevated in Groups II and III, in comparison to the control group. In this work, significant changes were found in the serum paraoxonase activity, HDL, TAS and TOS levels of fishes living in environments added 0.01 and 0.02 mg/L glyphosate.
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    Effects of nitrogen fertilizer on Capoeta capoeta: an immunohistopathological and biochemical investigation
    (Tubitak Scientific & Technological Research Council Turkey, 2022) Aydin, Seda Vural; Ozen, Hasan; Yilmaz, Muhitdin
    Increased use of fertilizers to increase plant production causes important environmental pollution not only in soil but also in water resources. Soil structure, water quality, water systems, and biodiversity are adversely affected by the unconscious use of fertilizers. In this study, Capoeta capoeta (Guldenstaedt 1773) were used to investigate the degenerative effects of nitrogen fertilizer. Twenty-four fish were equally allocated into three groups; group 1 (control), group 2 (15 mg/L nitrogen fertilizer in water) and group 3 (15 mg/L nitrogen fertilizer in water). Fish were exposed to the fertilizer for 15 days and then the tissues of gill, liver and intestine were investigated by immunohistopathological and biochemical means to assess the tissue degenerative effects of nitrogen fertilizers in water. Varying degrees of degenerative changes were observed in all of the investigated organs. In histopathological examinations, hydropic degeneration was observed in hepatocytes in fish exposed to fertilizer. Blunting and association in the gill tissue and occasional blunting in the intestinal tissue were detected. Catalase and SOD immunoreactivities increased in liver and gill tissues. There was also an increase in SOD immunoreactivity in the intestine of nitrogen fertilizer exposed fish. Total antioxidant status (TAS) also increased in both of the experimental groups (p < 0.05), but total oxidant status (TOS) did not change. The results of the experiment showed that nitrogen fertilizer in water resources may impair health status of fish by adversely affecting tissues in cellular level.
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    The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus)
    (2015) Karademir, Basaran; Koç, Evren; Ersan, Yusuf; Yilmaz, Muhitdin; Uslu, Hamit
    Bakır (II) Sülfat Toksikasyonunun Fare (Mus musculus) Karaciğer Histopatolojisi, Karaciğer Protein Elektroforezi ve Plazma Biyokimyası Üzerine EtkisiBu çalışmada bakır (II) sülfat toksikasyonunun ergin fare karaciğer morfolojisi, karaciğer protein elektroforezi ve plazma biyokimyasal bulguları üzerine etkileri araştırıldı. Toplam 21 fare 3 gruba bölündü. 5 gün süreyle intraperitonal olarak kontrol grubuna serum fizyolojik, çalışma gruplarına sırasıyla 2 ve 6 mg/kg bakır sülfat verildi. Çalışma sonunda, deneklerden kan ve karaciğer örnekleri toplandı. Bu örnekler üzerinde histopatolojik, elektroforetik ve biyokimyasal analizler yapıldı. Histopatolojik bulgulara göre doza bağlı olarak karaciğerde dejenerasyonlar tespit edildi. Karaciğer protein elektroforezinde kontrol grubuna kıyasla 2 mg/kg bakır sülfat grubunda protein bantlarında incelme, 6 mg/kg CuSO4 grubunda protein bantlarında ise kalınlaşma gözlemlendi. Biyokimyasal analizlerde plazma Total Oksidan Seviyesi (TOS) her iki grupta da istatistiksel olarak önemli düzeyde arttı (P0.05). Bununla birlikte plazma AST düzeyi yalnızca 6 mg/kg CuSO4 grubunda arttı (P0.01). Sonuç olarak, bakır sülfat toksikasyonunun farelerin karaciğerlerinin histopatolojisi ve karaciğer protein elektroforezi üzerinde negatif etkisi oldu. Fakat bu bulguları plazma biyokimyasal verileri tam olarak desteklemedi.
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    THE EFFECT OF DIMETHOATE ON CAPOETA CAPOETA (GULDENSTAEDT 1773) AND THE PROTECTIVE EFFECT OF ASCORBIC ACID
    (Parlar Scientific Publications (P S P), 2016) Tanriverdi, Erdi Anil; Yilmaz, Muhitdin; Koc, Evren; Akgul, Ali; Ersan, Yusuf
    In this study, Dimethoate (DM) (a pesticide)'s histopathological and electrophoretic effects were investigated on Capoeta capoeta (Guldenstaedt 1773) that were captured from Kars creek, and the protective effect of ascorbic acid (AA) (vitamin C) was tested. After allowing time for their adaptations to their new environment, the fish caught from Kars creek were divided into 4 groups (each including 10 fish), and placed in 300-liter tanks in the laboratory. The fish were kept in water containing different solutions: group I in normal water medium, group II in 100 mg/L AA, group III in 2 mg/L DM, and group IV in 100 mg/L AA and 2 mg/L DM. They were kept in these water mediums for 10 days. After this period, blood and tissue samples were taken from the fish for electrophoretic and histopathological studies. Tissue samples were detected in 10% formaldehyde solution and paraffin blocks were prepared by routine histological methods. The samples were cut into sections 3-5 thick, all stained according to hematoxylin and eosin staining methods, and they were examined under a microscope. Obtained serum samples were carried out in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). When we compared the serum samples with the control group of fish, protein bands in the application of 100 mg/L AA and 2 mg/L DM, thickening in 36 kD band, thinning in 100 kD, 97 kD, 76 kD, 56 kD and 30 kD bands were observed, and the bands of 93 kD, 46 kD, 44 kD and 26 kD were detected as newly-synthesized in SDS-PAGE electropherogram. In the histopathological examinations, depending on DM in intestine, liver, and kidney tissues obtained from the group formed by application of DM and AA, degeneration, necrosis, pyknotic nuclei and cell infiltrates were detected. As a result, whereas DM application was found to create toxic effects on Capoeta capoeta, AA was found to have a healing effect.
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    THE EFFECT OF TEBUCONAZOLE ON SERUM PARAOXONASE AND AMINOTRANSFERASE ACTIVITIES IN CYPRINUS CARPIO (L. 1758)
    (Parlar Scientific Publications (P S P), 2017) Nur, Gokhan; Yilmaz, Muhitdin; Karapehlivan, Mahmut; Kaya, Ivan; Nur, Ozlem; Deveci, Ayla
    The purpose of this study was to examine serum paraoxonase (PON1), alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and high density lipoprotein (HDL) levels of tebuconazole administered Cyprinus carpio (L. 1758). After providing adaptation of the fish in the laboratory setting for 7 days, they were divided into 3 groups, each of which included 8 fish. The fish in Group I were kept in normal water environment and those in Groups II and III were in 2 and 3 mg/L tebuconazole added water tanks, respectively, for 14 days. Blood samples were taken from the fish and serums were separated. Then, PON1, ALT, and AST activities and HDL levels were analyzed. While PON1 activity and HDL levels decreased in tebuconazole administered groups compared to Group I, increases were determined in terms of ALT and AST. Consequently, it was found that tebuconazole administrations caused significant changes in serum PON1, ALT, and AST activities and HDL levels of Cyprinus carpio.
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    The Effects of Carbaryl and 2,4-Dichlorophenoxyacetic Acid on Oxidative Stress Index in Capoeta capoeta (Guldensteadt 1773)
    (Zoological Soc Pakistan, 2019) Kaya, Inan; Yilmaz, Muhitdin; Kaya, Muge Mavioglu; Kukurt, Abdulsamed; Karapehlivan, Mahmut
    The aim of the present study is to investigate the oxidative stress index in Capoeta capoeta treated with carbaryl and 2,4-dichlorophenoxyacetic acid (2,4-D). Fifty Capoeta capoeta fish caught in Kars Creek were equally divided into five groups and acclimatized in separate tanks for 10 days. The fish were kept in tanks as follows: Group I (control) was kept in normal water. Group II, III, IV and V were kept in separate tanks containing 0.3 mg/L carbaryl, 0.6 mg/L carbaryl, 10 mg/L 2,4-D and 20 mg/L 2,4-D, respectively. The blood and liver samples were taken from the fish for biochemical studies at the end of the study period. Oxidative stress index (OSI) in plasma were evaluated as well as AST and ALT levels in the liver. Plasma OSI and liver AST levels in the groups with carbaryl and 2,4-D were compared with the control group, and they were found to be significantly higher. As a result, it was concluded that carbaryl and 2,4-D administered under of LC50 value caused liver damage by increasing the oxidative stress of Capoeta capoeta.
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    The Interaction Between Concentration of Heavy Metal-Trace Elements and Non-Smoking Status of Adolescents in Sinop (Turkey)
    (Humana Press Inc, 2020) Cabar, Huriye Demet; Ersoy Karacuha, Melek; Yilmaz, Muhitdin
    This study was experimentally conducted between 2017 and 2018 in order to determine the interactions of heavy metals and non-smoking status of adolescents in Sinop Province (Turkey). In this regard, the students of Sari Saltuk Anatolian High School students in Sinop Province were examined. The research data was obtained using a questionnaire, including questions about sociodemographic characteristics and smoking status of the adolescences. Afterwards, 0.5 g of hair samples from 40 female students who accepted to participate to the research was collected and analyzed by inductively coupled plasma mass spectrometry (ICP-MSMS), and the obtained data were evaluated using SPSS (version 22.0) statistical program. At the end of the study, the Mg, Cr, Co, and Zn elements of the non-smoking girls were found to be slightly higher than the normal range of hair, and the high concentration of Cd element was related to the contamination by passive smoking. Besides, it was determined that the elements which showed the highest positive correlation in hair samples taken from the children of smokers were Ca/Cd, Al/Fe, Mg/Cr, Na/Fe, Al/Cd, and Al/Na, respectively. As the nursing approach, it is thought that trainings aiming to prevent smoking and quitting smoking are extremely important.
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    The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)
    (2016) Yilmaz, Muhitdin; Koç, Evren; Atakişi, Onur; Harmankaya, Ahmet; Ersan, Yusuf; Karaman, Musa
    Çalışmada, Capoeta capoeta'da Kurşun (II) asetat (Pb(C2H3O2)2) toksisitesine karşı L-karnitinin (LK) koruyucu etkileri histopatolojik, elektroforetik ve biyokimyasal yöntemlerle incelendi. Kars Çayı'ndan yakalanan balıklar 500 L'lik tanklarda her grupta 10'ar adet balık bulunan 4 gruba ayrıldı. Kurşun ve sıvı LK suya ilave edildi. I. gruptaki balıklar kontrol grubu olarak belirlendi. II. gruptaki balıklara 10 gün süreyle 1 mg/L kurşun asetat uygulandı. III. gruptaki balıklara 100 mg/L LK uygulandı. IV. gruptaki balıklar ise 1 mg/L kurşun ve 100 mg/L dozunda LK uygulandı. Karaciğer, solungaç, bağırsak ve böbrek dokularında kurşun asetatın toksik etki gösterdiği ve buna karşı L-karnitin uygulamasının koruyucu etki göstererek oluşan bu dejenerasyonların şiddetini azalttığı gözlemlendi. Elektroforetik incelemede, kurşun asetatın birçok protein bandında inhibisyona neden olduğu, LK uygulaması sonucunda da protein ekspresyonlarında artış meydana geldiği saptandı. Kurşun uygulanan grupta total protein düzeyinin yüksek, kurşun LK verilen grubun ise düşük olduğu belirlendi (P0.05). Globulin düzeylerinin, LK kurşun verilen grupta istatistiksel olarak düşük olduğu saptandı (P0.05). Kurşun uygulanan grubun Total Oksidan Kapasitesinin (TOK) kontrol grubuna göre yüksek, kurşun LK verilen grubun TOK düzeyinin ise kontrol ile kurşun verilen grubun arasında olduğu belirlendi. Sonuç olarak; LK'in Capoeta capoeta'da kurşun toksisitesine karşı koruyucu özellik gösterdiği kanısına varıldı.
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    The relationship between dietary habits of late adolescent individuals and the heavy metal accumulation in hair
    (Mattioli 1885, 2020) Gonener, Ahmet; Karacuha, Melek Ersoy; Cabar, H. Demet; Yilmaz, Muhitdin; Gonener, Utku
    Study Objectives: This study aimed to determine the effects of smoking status and dietary habits on heavy metal accumulation in late adolescent students studying at Sinop University. Methods: This experimental research was conducted between 09 October and 15 November 2019. The population of the study was composed of 18-21 age (late adolescent period) students studying at Sinop University, School of Health. In the determination of the research sample, the selection of students who accepted to participate in the research as the case selection criteria, who did not have any mental or chronic disease/syndrome, and who did not use drugs continuously was taken into consideration. For the collection of research data, an information form about the weekly consumption of foods and smoking status was prepared by the researchers considering the geographical and physical locations where the students are located. After collecting the forms filled by students, 0.5 g hair samples were taken from volunteered 41 students to analyze the concentration of heavy metal in the hair and analyzed in the ICP-MS device. The data were evaluated using the Mann-Whitney U test in the SPSS (version 22.0) statistical program. Results: As a result of this research, it has been revealed that there was a statistically significant relationship between some food groups (lettuce, chips, instant soup, salami, and trout) that students express at least once a week and the heavy metal concentration detected in their hair. Conclusion: It was revealed that there is a relationship between individuals' dietary habits and heavy metals in late adolescence.