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Öğe n-3 LC-PUFA Enrichment Protocol for Red Earthworm, Eisenia fetida: A Cheap and Sustainable Method(Central Fisheries Research Inst, 2021) Kumlu, Metin; Beksari, Asuman; Yilmaz, Hatice Asuman; Sariipek, Merve; Kinay, Enes; Turchini, Giovanni M.; Eroldogan, Orhan TufanThis study assessed the potential of omega-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) enrichment in red earthworm (Eisenia fetida, REW), via the addition of fish oil (FO, anchovy oil) to the culture-compost. FO addition to compost was tested at graded inclusion doses (0, 2.5, 5 and 10 g/100 g compost) and for different time-periods, up to 96 h. Dose, time, as well as dose and time interactions of FO compost enrichment had significant effects on all fatty acids of earthworm bodies. The enrichment with 10 g FO/100 g compost sharply increased DHA levels of the worms (14.01 mol%) within just 24 h, compared to the control group (0.92 mol%). Similar increases (ranging between 10.99 and 15.55 mol%) occurred only after 48 and 96 h in lower FO enrichment levels (2.5 and 5.0 g/100 g compost, respectively). Therefore, it was concluded that, to obtain maximum n-3 LC-PUFA enrichment efficiency in REW, an enrichment period of 24 h is ideal and recommended for the 10 g FO/100 g compost, whereas longer enrichment periods (48-96 h) are more suitable for lower levels of FO inclusions (2.5 or 5 g/100 g compost).Öğe Response of Fatty Acid Composition of the Green Tiger Shrimp Penaeus semisulcatus During the Overwintering Period(Central Fisheries Research Inst, 2019) Kumlu, Metin; Kinay, Enes; Yilmaz, Hatice Asuman; Beksari, Asuman; Eroldogan, Orhan Tufan; Sariipek, MerveResponse of proximate and fatty acid (FA) composition of the green tiger shrimp Penaeus semisulcatus in relation to changing water temperatures during over wintering (for 8-weeks at 11-16 degrees C) and then refeeding periods (at 28 degrees C for a further 2-weeks) was investigated in this study. The shrimps did not appear to need to catabolise their either body proteins or lipids during the overwintering period, as the feeding was not ceased completely. While muscle protein, ash and dry matter compositions of the shrimps did not change by changing temperature, lipid increased from 1% during the overwintering period to 1.2% during the recovery period (P<0.01). Saturated FAs (SFA), mono-unsaturated FAs (MUFA), and partially poly-unsaturated FAs (n-6 PUFA) declined, on the contrary, n-3 PUFA significantly rose in the muscle and hepatopacreas during the cold exposure. Shrimps tended to consume especially SFA and, to a lesser degree, MUFA under sub-optimal conditions (P<0.01). PUFA and LC-PUFA appeared to be selectively retained or even elevated in the shrimp muscle during the cold season. Over wintering strategy might generate additional benefit for a heathier food supply for human nutrition, as shrimps can be enriched for LC-PUFA, by simply keeping them under cold water temperatures (10-15 degrees C) during overwintering period, with minimal cost of feeding.