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Öğe Comparıson of cumulus cells and follıcular fluıd obtaıned from ınfertile ındıvıduals dıagnosed wıth polycystıc ovary syndrome (PCOS) and endomethrıosıs wıth samples obtaıned from healthy ındıvıduals(2023) Dayanır, Duygu; Ruso, Halil; Kalem, Ziya; Tural, Rabia; Saribas, Gulistan Sanem; Dincel, Aylin Sepici; Gürgan, TimurAim: Investigating the relationship between Growth differentiation factor-9 (GDF-9), Bone morphogenetic protein-15 (BMP-15) markers, apoptosis levels in cumulus cells and total oxidant (TOS)/ anti-oxidant (TAS) stress levels, inflammation parameters (interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha)) in follicular fluid belonging to patients with polycystic ovary syndrome (PCOS), endometriosis (END) and male factor (MF) (control) groups. Material and Methods: GDF-9 and BMP-15 markers are determined by immunohistochemical methods, apoptosis levels are studied with TUNEL. TOS and TAS statuses are investigated with spectrophotometry, IL-6 and TNF – alpha levels are examined by Enzyme-Linked Immuno Sorbent Assay (ELISA). Results: According to the data obtained in the study; GDF-9 and BMP-15 levels are found to be lower in PCOS and END groups and apoptosis levels of cumulus cells were significantly higher at these groups . TOS levels were significantly higher in PCOS and END groups whereas follicular fluid TAS levels were not statistically significant for these groups. IL-6 and TNF – alpha levels of follicular fluid was significantly higher in PCOS. These parameters were also higher for END group, however the difference was not found to be significant. Conclusion: Our results imply that correlation between GDF-9, BMP-15 markers, apotosis levels, oxidative status, inflammation levels may be interpreted with improper environment for oocyte maturation for patients diagnosed with PCOS or END. The need for further studies on subject proceeds. However, if similar datas are obtained in further studies, it is thought that evaluation of cumulus cell properties together with especially follicular fluid oxidative stress levels will contribute to the selection of the best oocyte.Öğe Effect of Resveratrol and Quercetin on Intestinal Ischemia Reperfusion Injury in Rats(2023) Yağmur, Işıl; Dindar, Selen; Tural, Rabia; Yaylalı, Aslı; Yoldas, Atila; Tolun, Fatma Inanc; Daglı, HasanAim: Intestinal ischemia reperfusion (I/R) injury is an emergency condition with a high mortality rate and early diagnosis is very difficult. In this study, we aimed to examine the biochemical and histopathological effects of resveratrol and quercetin on intestinal I/R injury model. Material and Method: In our study, 56 male Sprague-Dawley rats weighing 250-300 g were randomly divided into 7 groups consisting of 8 rats. Groups were control group (group 1), saline group (group 2), ethanol group (group 3), resveratrol group (group 4) (30mg/kg), quercetin group (group 5) (30mg/kg), resveratrol+quercetin group A (group 6) (15 mg/kg+15 mg/kg), resveratrol+quercetin group B (group 7) (30 mg/kg+30 mg/kg). At the end of the experiment rats intestinal tissues were divided into 2 parts for biochemical and histopathological examination. Total oxidant level (TOS), total antioxidant level (TAS), total thiol [(-SH)+(-S-S-)] (TT), native thiol [-SH] (NT), and protein content levels were measured spectrophotometrically, oxidative stress index (OSI) and disulfide [-S-S-] levels were calculated. Results: A statistically significant difference was found between the groups in terms of TOS, OSI, TT, NT and disulfide levels (pÖğe Environmental pollutant sodium omadine: toxic effects in zebra fish (Danio rerio)(Taylor & Francis Ltd, 2024) Yilmaz Sezer, Ilknur; Kocak, Gulsum; Tural, Rabia; Genal, Aysel caglan; Sepici Dincel, AylinIn recent years one of the most striking results of over-population and consumption activities in the world is the rapid increase in environmental pollutants. Environmental pollutants, one of the harmful consequences of technological and modern life, threaten the health of people and other living organisms. In this study, we aimed to determine the effects of sodium omadine (NaOM) on superoxide dismutase enzyme (SOD) activity as an antioxidant and on 8-OHdG levels as oxidative DNA damage in zebrafish. Zebrafish, obtained from the aquarium fish producer, were stocked in experimental aquariums to ensure their adaptation period to the experimental conditions 15 days before the experiment. The fish were exposed to 1 ug/L and 5 ug/L concentrations of NaOM for 24, 72, and 96 h. SOD enzyme activity (U/100 mg tissue) and 8-OHdG (pg/100 mg tissue) were measured using commercial kits. The statistically significant differences in tissue SOD levels and data for DNA damage between the groups were determined as time and dose-dependent (p < 0.05). Biocidal products are environmental pollutants that cause changes in antioxidant enzyme activities, especially in non-target organisms. Marine pollution and the degradation of ecosystems directly affect people, and the results of the study offer awareness of health problems, environmental pollution, and marine pollution.Öğe Evaluation of Oxidative Stress Parameters in Male Textile Workers Working in Different Sections of Production(Kahramanmaras Sütçü Imam Üniversitesi, 2024) Dokumaci, Leyla; Yagmur, Isil; Tural, Rabia; İnanç Tolun, Fatma; Dagli, Hasan; Doboglu, Yeliz; Sager, HaticeObjective: Occupationally exposed environmental conditions and many substances threaten human health by causing diseases. This study examines biochemically how the oxidant and antioxidant system parameters change in the employees working in different textile departments.Material and Methods:3 groups of 30 people were formed, each consisting of healthy men who do not smoke or drink alcohol, working in the office, dyehouse and yarn departments of a textile company in Kahramanmaras office worker (control group, group 1), dyehouse worker (group 2), yarn worker (group 3). After 12 hours of fasting, venous blood samples were taken and serum samples were separated. Total oxidant status (TOS), total antioxidant status (TAS), total thiolÖğe Evaluation of Rheum ribes L. Protective Effect with G Protein-Coupled Estrogen Receptor-1 (GPER-1) Levels in Experimental Liver Ischemia-Reperfusion Model(2023) Yağmur, Işıl; Kurutas, Ergul Belge; Yuzbasioglu, Mehmet Fatih; Bakaris, Sevgi; Çitil, Büşra; Doğaner, Adem; Tural, RabiaObjective: To evaluate the relationship between the cell protective effects of Rheum ribes L. and G protein-coupled estrogen receptor-1 (GPER-1) levels in ischemia-reperfusion (I/R) injury. Material and Method: The 32 male Wistar-Albino rats we used in this study were randomly divided into 4 groups of 8 rats each; control group (group 1), sham group (group 2), I/R group (group 3), I/R+Rheum ribes L. group (group 4). While no procedure was applied to the control group, 30 minutes of ischemia followed by 30 minutes of reperfusion was applied to the rats in all other groups. GPER-1 levels in liver tissue were measured with an ELISA reader. Histopathological examination of the tissues was performed under light microscopy. Results: As a result of biochemical analysis; GPER-1 levels were statistically significantly decreased in the sham and I/R groups compared to control and I/R+Rheum ribes L. groups; in the I/R+Rheum ribes L. group compared to the control group (p <0.05). In the histopathological examination of the liver, necrosis and congestion observed in the Sham and I/R groups were significant when compared to the control group. While vacuolization was observed in a few experimental animals, there was a significant difference in the sham and I/R groups compared to the control group (p ˂0.05). I/R+Rheum ribes L. group showed improvement in histopathological criteria in terms of vacuolization and necrosis compared to the sham group and I/R groups, and the difference was significant. (p ˂0.05). Conclusion: Rheum ribes L. can protect hepatocytes both with its antioxidant effects and GPER-1 activation.
