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Öğe A study of three bacteria isolated from marine sediment and description of Micromonospora globispora sp. nov.(Elsevier Gmbh, 2019) Carro, Lorena; Veyisoglu, Aysel; Cetin, Demet; Igual, Jose M.; Klenk, Hans-Peter; Trujillo, Martha E.; Sahin, NevzatDuring a study looking for the isolation of new actinobacteria strains with potential for antibiotic production from deep marine sediment, three strains were collected with a morphology similar to the one described for the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of the strains S2901(T), S2903, and S2904. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, meso-diaminopimelic acid in the wall peptidoglycan, a tetrahydrogenated menaquinone with nine isoprene units as major respiratory quinone, iso-C-15:0 and iso-C-16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strain S2901(T), S2903, and S2904 showed the highest similarity (99.2%) with the type strain of Micromonospora halophytica DSM 43171(T), forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with gyrB gene and MLSA phylogenies. Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the isolates should be assigned to a new species within the genus Micromonospora for which the name Micromonospora globispora sp. nov. (S2901(T), S2903 and S2904) is proposed. (C) 2018 Elsevier GmbH. All rights reserved.Öğe Micromonospora phytophila sp nov and Micromonospora luteiviridis sp nov., isolated as natural inhabitants of plant nodules(Microbiology Soc, 2018) Carro, Lorena; Veyisoglu, Aysel; Riesco, Raul; Sproeer, Cathrin; Klenk, Hans-Peter; Sahin, Nevzat; Trujillo, Martha E.Two actinobacterial isolates, strains SG15(T) and SGB14(T), were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora. For strains SG15(T) and SGB14(T), the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248(T) (99.2 %) and Micromonospora purpureochromogenes DSM 43821(T) (99.4 %), respectively. However, strains SG15(T) and SGB14(T) were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15(T) (=CECT 9369(T); =DSM 105363(T)), and Micromonospora luteiviridis sp. nov. type strain SGB14(T) (=CECT 9370(T); =DSM 105362(T)).