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    Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
    (Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoglu, Aysel; Tokatli, Ali
    Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
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    Streptomyces boluensissp. nov., isolated from lake sediment
    (Springer, 2020) Tokatli, Ali; Idil, Onder; Veyisoglu, Aysel; Saygin, Hayrettin; Guven, Kiymet; Cetin, Demet; Sahin, Nevzat
    A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537(T), was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genusStreptomyces. 16S rRNA gene sequence analysis of strain YC537(T)showed that it is closely related to the type strain ofStreptomyces ziwulingensisF22(T)(97.9% sequence similarity),Streptomyces tauricusJCM 4837( T)(97.7%) andStreptomyces beijiangensisNBRC 100044( T)(97.6%). The cell wall of the strain containedLL-diaminopimelic acid and the cell-wall sugars were glucose, galactose and ribose. The major phospholipids of strain YC537(T)were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H-6) and MK-9(H-8). The major cellular fatty acids wereiso-C-16:0,iso-C-14:0,anteiso-C(15:0)andiso-C-15:0. Consequently, strain YC537(T)is considered to represent a novel species in the genusStreptomyces, for which the nameStreptomyces boluensissp. nov. is proposed. The type strain is YC537(T)(= KCTC 39750( T) = DSM 102303( T)).
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    Streptomyces coryli sp. nov., isolated from hazelnut orchard soil
    (Microbiology Soc, 2020) Saygin, Hayrettin; Veyisoglu, Aysel; Tatar, Demet; Nigiz, Cengiz; Tokatli, Ali; Sahin, Nevzat
    A novel actinobacteria, isolate A7024(T), was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 165 rRNA gene sequences indicated that isolate A7024(T) was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7%) to Streptomyces cadmiisoli ZFG47(T). The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024(T) contained LL-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol. phosphatidylethanol-amine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H-6) and MK-9(H-4). The major cellular fatty acids were iso-C-16:0, anteiso-C-1(5:0) and anteiso-C-1(7:)0. Strain A7024(T) had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 +/- 2.3% digital DNA-DNA-hybridization and 76.94% average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024(T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024(T) (=DSM 42066(T)=KCTC 29102(T)=NRRL B-24888(T)).