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    ALPHA-TOCOPHEROL-DEPENDENT SALT TOLERANCE IS MORE RELATED WITH AUXIN SYNTHESIS RATHER THAN ENHANCEMENT ANTIOXIDANT DEFENSE IN SOYBEAN ROOTS
    (Akademiai Kiado Rt, 2017) Sereflioglu, Seda; Dinler, Burcu Seckin; Tasci, Eda
    In this paper, we describe the alleviated effects of alpha-tocopherol (alpha-T) on oxidative damage and its possible role as a signal transmitter in plants during salt stress. The results show that exogenously applied alpha-T under salt stress increased root length and weight, but reduced hydrogen peroxide (H2O2), superoxide anion radical (O-2 center dot(-)) and malondialdehyde (MDA) content in soybean roots. The proline content was reduced by alpha-T treatment. Interestingly, endogenous auxin (IAA) level was significantly increased after alpha-T application as compared to salt stress alone. Moreover, alpha-T reduced significantly superoxide dismutase (SOD) enzyme and isoenzyme activity but upregulated peroxidase (POX) 2, 3 and glutathione-stransferase (GST) 1, 3 isoenzyme expression. However, ascorbate peroxidase (APX) enzyme activity was not affected at all. Consequently, the results show that alpha-T serves as a signal molecule under salinity from leaves to roots by increasing remarkably endogenous IAA levels and increasing partially antioxidant activity in roots.
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    DEHYDROABIETIC ACID MEDIATED WRKY71 GENE EXPRESSIONS AND REACTIVE OXYGEN SPECIES REGULATION IN SOYBEAN UNDER SALINITY
    (Polska Akad Nauk, Polish Acad Sciences, Pas Branch Cracow, 2020) Tasci, Eda; Dinler, Burcu Seckin
    Dehydroabietic acid R=COOH (DHA), a naturally occuring diterpene resin acid, is an activator of systemic acquired resistance (SAR) under biotic stress. However, there had been no report on its functioning under salinity. In the present study, we determined the effects of DHA on salinity and its possible role as a signal transmitter in soybean (Glycine max L.) leaves under salinity (200 mM NaCl). Furthermore, physiological parameters, chlorophyll, proline, malondialdehyde (MDA), hydrogen peroxide (H2O2) content, superoxide (O-2(-)) and hydroxyl radical ((OH)-O-center dot) scavenge capacity, as well as antioxidant enzymes (SOD, POX, APX and GST) and GmWRKY-71 gene expressions were investigated in the treated plants at 6 h, 12 h and 24 h. The obtained results showed that pretreatment of DHA caused (1) a reduction in salt-induced damage, (2) improvement in biomass yield, water status, chlorophyll and leaf area, (3) regulation of the proline level and relative electrolyte leakage, (4) increase in reactive oxygen species (ROS) scavenging capacity, (5) induction of SOD and APX enzyme activity at all the investigated periods, while POX only at 6 h, and thus alleviation of the oxidative damage. In addition, the changes in GmWRKY-71 gene expressions were remarkable in soybean under salinity. To sum up, these results showed that DHA can be used as a ROS inhibitor or a signal molecule in increasing salt tolerance in soybean under salinity.
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    Guano-induced germination and responses of wheat seedlings to guano under water stress treatments
    (2013) Tasci, Eda; Seckin, Burcu Dinler
    Bu çalışma organik bir gübre olan guanonun buğday (Triticum durum L. Karakılcık) bitkisinin çimlenme ve büyüme parametreleri üzerine olan etkisini tespit etmek amacı ile yapılmıştır. Guano çimlenme ortamına üç gün boyunca 6g /100 ml oranında uygulanmıştır.Çimlenmenin ardından bir grup yedi gün boyunca su kıtlığına maruz bırakılıp diğer bir grup düzenli olarak sulanmıştır. Çimlenme oranı ve çimlenen tohumlardaki kuru ağırlık guano uygulanan grupta daha yüksek bulunmuştur.Vejetatif aşamada guano uygulamaları su stresi altında bağıl su içeriği ve bağıl büyüme oranını geliştirmiştir.Su stresi malondialdehid ve hidrojen peroksit (H2O2) içeriğini arttırmış fakat bu parametreler su stresi ve guano uygulamalarının birlikte yapıldığı grupta belirgin bir değişikliğe uğramamışlardır.Fide aşamasında aba miktarı guano ve su stresi grubunda azalmış fakat guano uygulaması su stresi altında ABA (absisik asit) miktarını arttırmıştır. Bu sonuçlara göre guano uygulamasının buğday tohumlarında çimlenme ve büyüme parametrelerini iyileştirdiği ayrıca buğday fidelerinin su stresi altında oluşan oksidatif hasardan koruduğu ortaya konulmuştur.
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    Investigation of Comparative Regulation on Antioxidant Enzyme System under Copper Treatment and Drought Stress in Maize (Zea mays L.)
    (Univ Agr Sci & Veterinary Med Cluj-Napoca, 2014) Cetinkaya, Hatice; Seckin Dinler, Burcu; Tasci, Eda
    The present study was conducted to present the responses of drought-sensitive 'Shemal' and drought-tolerant '71MAY69' maize cultivars under drought condition (20% Polyethylene glycol, -0.40 MPa) and three different copper concentrations (0.5 mM, 1 mM, 1.5 mM CuSO4 center dot 5H(2)O) for 5 days to determine the enzymatic responses of copper treatment in maize leaves. Copper treatments alone did not change stomata! conductance, relative water content, malondialdehyde, proline, hydrogen peroxide content and abscisic acid level according to control groups. Combined treatment (drought and copper) alleviated the damage of PEG- induced drought stress in maize leaves. Superoxide dismutase (SOD), catalase (CAT), glutatione reductase (GR) activity increased and glutathione -S transferase (GST) activity decreased, while ascorbate peroxidase (APX) activity did not change under drought stress in the tolerant cultivar. SOD, CAT and APX were decreased and GST activities were increased while GR did not change in 'Shemal'. Also SOD, APX and CAT activity increased by copper treatment alone in both cultivars. Otherwise combined treatment increased SOD, APX and CAT activity at all concentrations, but GR and GST activity increased only by (PEG+1.5 mM) treatment when compared with PEG treatment alone in sensitive ones. As a result, exogenous copper alleviated drought stress, while it induced an oxidative damage by increasing antioxidant enzyme activities differently from drought tolerance. Copper tolerance in maize is not a common response of its defense mechanism because of different response to copper and drought in the same cultivar.
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    THE COOPERATION BETWEEN METHYL JASMONATE AND SALICYLIC ACID TO PROTECT SOYBEAN (GLYCINE MAX L.) FROM SALINITY
    (Parlar Scientific Publications (P S P), 2018) Seckin-Dinler, Burcu; Tasci, Eda; Sarisoy, Ufkun; Gul, Volkan
    Methyl jasmonate (MeJA), a methyl ester of jasmonic acid, is a naturally produced phytohormone that regulates plant growth and development by maintaining the morphologic, physiological, and biochemical development of plants. The purpose of this study was to determine the effect of methyl jasmonate and endogenous salicylic acid on the stimulation and scavenging of reactive oxygen species (ROS) under salt stress in soybean roots and leaves. Soybean leaves were sprayed with 0.5 mu M methyl jasmonate for 4 days, followed by exposure to 200 mM NaCl. Foliar application of MeJA did not change the leaf area; however, it increased the relative water content (RWC), chlorophyll content, relative electrolyte leakage (REL), and root and shoot fresh weight, and it lessened the stress-induced oxidative damage by decreasing the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA). Curiously, catalase (CAT) enzyme and isoenzyme activity, and also endogenous salicylic acid (SA) were decreased by MeJA treatment. As a result, this study firstly determined that MeJA treatment improved the soybean performance under salinity by acting role as an antioxidant and cooperating with SA.
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    The Effects of NPR1 Dependent Salicylic Acid Change in Increasing Salt Tolerance of Soybean Leaves by Acclimation
    (Univ Agr Sci & Veterinary Med Cluj-Napoca, 2018) Sarisoy, Ufkun; Seckin Dinler, Burcu; Tasci, Eda
    Non-expressor of Pathogen Related 1 (NPR1) is a regulatory gene of the salicylic acid (SA) signaling pathway, the detailed mechanism of which is still not well understood. This study investigated the effects of NPR1-dependent SA level change on increasing salt tolerance of soybean leaves with acclimation. Salt-sensitive ('SA88') and salt-tolerant ('Erensoy') soybean (Glycine max L.) plants were treated with increasing NaCl concentrations (25, 50, 75, and 100 mM; acclimation) and with 100 mM NaCl directly (non-acclimation) in two groups. The results showed that acclimation treatment alleviated salt-induced damage in the sensitive cultivar with increasing superoxide anion radical scavenging activity, and decreasing hydrogen peroxide (H2O2) and malondialdehyde (MDA) content. However, the APX, CAT, and GST enzyme activities were increased by acclimation treatment, with the highest increase observed in GST enzymes. Interestingly, the Gmnpr1 gene expression was upregulated in all treatments but was more pronounced in non-acclimation. Furthermore, the highest increase in endogenous SA level was under acclimation treatment in 'SA88'. In conclusion, the results firstly showed that an acclimation process is useful for increasing salt tolerance in sensitive soybean plants with only ROS-inducted NPR1-independent SA accumulation but not through the NPR1-dependent SA signaling pathway.