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    COMPARATIVE ANALYSIS OF BIOCHEMICAL CONTENT, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF HYPERICUM PERFORATUM L. SPECIES IS GROWN IN T?RK?YE
    (Pakistan Botanical Soc, 2023) Gul, Volkan; Cetinkaya, Hatice; Dinler, Burcu Seckin; Sefaoglu, Firat
    Many external factors, such as climatic conditions, geographical differences, and altitude, directly affect the primary and secondary metabolites and therapeutic use of St. John's Wort (Hypericum perforatum L.), an important plant in alternative medicine. This study was carried out to identify the antioxidant and antimicrobial activities and the content of proline, malondialdehyde, hydrogen peroxide, flavonoid, anthocyanin, and total phenolic in the St. John's Wort growing naturally in different regions of Bayburt. The St. John's Wort samples were collected from 11 different locations. As a result of the analyses carried out on the plant samples, it was found that the content of proline was within the range of 4.6-8.2 mu mol g-1 DW, the amount of malondialdehyde was within the range of 0.69-1.10 nmol g-1 DW, the amount of hydrogen peroxide was within the range of 79.89-155.49 mu mol g-1 DW, the amount of flavonoids was within the range of 91.38%100.27%, the amount of anthocyanin was within the range of 0.15-1.11 mkmol g-1 DW, the total phenolic content was within the range of 15.82-45.22 mg GAE g-1, and the antioxidant activity was within the range of 6.07-105.60 mu g mL-1.In conclusion, although the biochemical contents and nonenzymatic antioxidant activities showed a wide variety, the antioxidant activity was found to be higher in samples 5, 8, and 11. According to the antimicrobial activity results, some plant extracts were effective on bacteria and fungi, especially samples 5, 6, 8, and 11 showed an effect at the concentration of 200 mu g mL-1. This study, the first regional study on this subject, will contribute to future research and clinical trials in the health field.
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    Effects of titanium dioxide nanoparticles against salt and heat stress in safflower cultivars
    (Soc Botanica Brasil, 2024) Dinler, Burcu Seckin; Cetinkaya, Hatice; Koc, Fatma Nur; Gul, Volkan; Sefaoglu, Firat
    This study aimed to determine the effects of titanium dioxide nanoparticle (TiO2NP) pretreatment on seeds of different safflower cultivars (Balci, Dincer) under salt and heat stresses. The apparent effects on stress markers (malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide radical (O2 center dot-) content), as well as changes in germination and physiological parameters (radicle and plumula weight and length measurements), were investigated. TiO2NP pretreatment caused an increase in radicle length and plumula fresh weight for the Balci cultivar under salinity. Furthermore, plumula dry weight was alleviated with TiO2NP pretreatment for both cultivars. TiO2NP pretreatment improved plumula dry and fresh weights for both cultivars under heat stress. In addition, MDA content decreased for both cultivars under heat stress but only for Balci under salt stress. The amount of O2 center dot- radicals positively affected only the radicle for both cultivars under heat stress. This study is the first to document the alleviation of salt stress damage for the Balci safflower cultivar, and protection for both Balci and Dincer cultivars under heat stress, using 200 ppm TiO2NP pretreatment.
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    The Effect of Different Doses of Salt Stress on Germination and Emergence in Cannabis ( Cannabis sativa L.) Seed Treated with Pre-Salicylic Acid
    (Inst Tecnologia Parana, 2024) Gul, Volkan; Sefaoglu, Firat; Cetinkaya, Hatice; Dinler, Burcu Seckin
    It is known that salinity stress, one of the abiotic stress factors, significantly limits the germination, growth, development, and crop yield of plants. Therefore, this study was conducted to evaluate the effects of salinity (control, 50, 100 ve 150 mM) on seed germination and the effect of salicylic acid (control, 0.25, 0.50, 0.75 ve 1.00 mM) on seed germination to improve salt tolerance in cannabis seeds. The physiological characteristics of the plant, such as germination percentage, germination duration, germination index, radicle length, plumule length, wet radicle weight, dry radicle weight, wet plumule weight, and dry plumule weight, were analyzed. Depending on the salinity application dose, it was observed that there was a significant decrease in the germination parameters of cannabis seeds compared to the control. It was observed that salicylic acid treatments under salinity stress positively affected all the characters examined and reduced germination arrest due to increasing concentration levels. Although it is recommended to prepare seeds with 0.5 mM salicylic acid pre-application dose against salt stress of the cannabis plant,it is essential to expand the studies on its transfer to practice. As a result, salicylic acid will provide positive results that can be transferred to practice by increasing the resistance of cannabis plants against salinity, especially in agricultural soils with salt problems, as it will be more sensitive to pests and diseases.