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Öğe Production of Rhizopus oryzae by solid state fermentation and evaluation of its biocomposite characteristics(Taylor & Francis Inc, 2026) Ozgul, Sevim; Duzel, Ahmet; Kocar, Gunnur; Sargin, SaitRhizophus oryzae biomass (RB) can be incorporated into polylactic acid (PLA) to create improved biocomposites that perfectly fit the biobased circular economy model. In this study, Rhizopus oryzae EGE-F75, produced through solid-state fermentation in aluminum trays with the maximum RB production conditions (Initial moisture (%): 79.84%, Inoculum ratio (%): 28.01, Incubation time (days): 9.74) was used as reinforcement material in PLA biocomposites. Biocomposites were obtained by adding varying amounts of RB (5% and 10% by weight) to PLA using a twin screw extruder, and the physical, thermal, mechanical and morphological properties of the biocomposites were investigated. According to the results, the incorporation of RB resulted in a decrease in mechanical properties. The tensile strength of 10% RB/PLA composite decreased by 23%, while the flexural modulus value of the composite increased by 6% compared to PLA. Furthermore, the addition of RB to PLA delayed the thermal decomposition of PLA and reduced its thermal conductivity. Introducing 5 wt% of RB to the PLA matrix reduced the thermal conductivity of the PLA matrix by 79%. Therefore, RB may not serve as an ideal filler for enhancing the mechanical properties and thermal stability of biocomposites, but it does offer potential as an alternative material for thermal insulation.Öğe Production of xylanase intended to be used in bread-making: laboratory scale and pilot scale studies(Taylor & Francis Ltd, 2024) Ongen, Gaye Ongen; Sahinbas, Dilek; Duzel, Ahmet; Duzdemir, Gizem Ezgi; Altinel, Burak; Tuluk, Kubra; Sargin, SaitIn this study, submerged and solid-state fermentation studies were performed for xylanase production simultaneously to determine ideal carbon and nitrogen sources together with necessary production parameters from the laboratory scale to the pilot scale. The results showed that barley malt sprout is effective as a nitrogen and a good carbon source. Under optimum conditions, xylanase activities 71.74 +/- 2.70 U/mL, 56.95 +/- 3.6 U/mL, 65.8 +/- 8 U/mL and 46.05 +/- 0.1 U/mL were achieved in shake flasks, 2, 10 and 30 L bioreactors, respectively. In solid-state fermentation studies, maximum activity in flasks was 759.15 +/- 36.16 U/g production media, and maximum activity in pilot-scale tray bioreactor productions was 1312 +/- 137.85 U/g dry substrate. Bread-making trials showed that using xylanase at 100 U/100g flour resulted in a significant increase in the loaf volume of wheat flour bread and whole wheat flour bread.












