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    Sprint and Anaerobic Power with the Soccer-Specific ACTN3 Gene: A Distintive Example
    (Soc Chilena Anatomia, 2024) Soyler, Mehmet; Zileli, Raif; Ozkamci, Huseyin; Diker, Gurkan; Sever, Mehmet Onur; Bayrakdaroglu, Serdar; On, Sadi
    The aim of this study is twofold: (1) to identify differences in certain anaerobic parameters (10m sprint, 30m sprint, anaerobic power, and Illinois agility tests) between professional and amateur soccer players, and (2) to determine whether there is a difference in the ACTN3 gene polymorphism between professional and amateur soccer players. Ultimately, the goal is to reveal which parameters contribute to the differentiation in these two aspects. A total of 133 volunteer soccer players, including 71 professionals and 62 amateurs, participated in the research. DNA extraction from buccal epithelial cells was performed using a commercial kit to determine the genetic background of the athletes, and Real -Time PCR was conducted for genotyping. Statistical analysis of the findings obtained from the test results was performed using the SPSS 23 (SPSS Inc., Chicago, IL, USA) package program. The homogeneity of variance of the data was assessed using the Levene Test, and normal distribution analyses were conducted using the Shapiro -Wilk Test. Chi-square and Mann -Whitney U tests were employed for parameter analysis. The significance level was set at p<0.05. Evaluation of the data in our study revealed no statistically significant difference in ACTN3 rs1815739 gene polymorphism between the groups (p>0.05). However, there is a statistically significant difference in anaerobic parameters (10m sprint, 30m sprint, and anaerobic power) except for the Illinois test (p<0.05). In conclusion, our study found that gene polymorphism is not a differentiating factor between professional and amateur soccer players, but speed (10m and 30m) and anaerobic power parameters are differentiating factors.
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    The Distinguishing Factor in Soccer Players is Aerobic Performance, not the ACTN3 Gene
    (Soc Chilena Anatomia, 2023) Zileli, Raif; Soyler, Mehmet; Diker, Gurkan; Ozkamci, Huseyin; Bayrakdaroglu, Serdar; Sever, Mehmet Onur; Can, Ibrahim
    The purpose of this study was to reveal the differences between ACTN3 genotype (RR, RX, XX) and aerobic performance [Yo-Yo IRT1 (m), VO2 max (ml/kg/min)] in professional and regional amateur league soccer players and to reveal which of these parameters was a distinctive factor in these athletes.71 professional soccer players (age: 23.66 +/- 4.11 years; body height: 1.79 +/- 6.99 m; body weight: 76.02 +/- 6.76 kg; body fat: 11.59 +/- 3.11 %) and 62 regional amateur soccer players (age: 23.63 +/- 3.77 years; body height: 1.81 +/- 5.77 m; body weight: 76.36 +/- 7.53 kg; body fat: 15.60 +/- 4.65 %) volunteered for the study. After DNA extraction from buccal epithelial cells via a commercial kit was performed for the genetic background of the athletes, Real-Time PCR was carried out for genotyping. Furthermore, Yo-Yo IRT1 test was performed to determine the aerobic performance of the soccer players. SPSS 23 (SPSS Inc., Chicago, IL, USA) package program was used for the statistical analysis of the data obtained in the tests. Shapiro-Wilk test for normality and Levene's test for homogeneity of variance were performed. Chi-Square, Independent Sample T Test and One Way ANOVA test were used in the analysis of the parameters. Statistical significance was set as p<0.05. In the study, there found no meaningful statistical significance in terms of the rs1815739 polymorphism in the ACTN3 gene between the groups (p>0.05); however, there was a statistical significance in favor of professional soccer players in terms of aerobic parameters (p<0.05). Consequently, it can be said that aerobic performance is the distinguishing factor, not the ACTN3 gene, in soccer players.