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    Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid
    (Cukurova Univ, Fac Medicine, 2021) Alizade, Ares; Ozbolat, Guluzar
    Purpose: The aim of this study was to investigate the effects of Inula viscosa extract and Curcumin on the U87 (human astrocytoma cell line) treated with amyloid-beta (A beta), which is the Alzheimer's disease (AD) model cell line. Materials and Methods: Firstly, the cytotoxic potential of inula and curcumin was investigated in the U87 cells by the colorimetric MF1 (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay. Then, the amount of Total Glutathione, Malondialdehyde (MDA), Glutathione reductase (GR) activities were investigated. ELISA test was used to examine the expression and activity of cleaved Bax and Bcl-2 proteins in the Inula viscosa and Curcumin treated U87 cell lines. Results: Inula viscosa and Curcumin treatment reduced cell death caused by amyloid-B in cells. It also reduced oxidative stress caused by amyloid-B, while reducing the activation of the proapoptotic protein Bax, and Bcl-2. Conclusion: Our results suggest that inula viscosa may represent a new approach in the treatment of Alzheimer's.
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    Effects of Curcumin on Iron Overload in Rats
    (Knowledge E, 2021) Ozbolat, Guluzar; Yegani, Arash Alizadeh
    Background: Iron overload, common in patients with hematological disorders, is a key target in drug development. This study investigated the effects of curcumin on iron overload in rats. Methods: Forty male Wistar rats weighing 139.78 +/- 11.95 gm (Mean +/- SD) were divided into three equal groups: (i) controls; (ii) iron overload group that received six doses of iron dextran 1000 mg/kg(--1) by intraperitoneal injections (i.p.); and (iii) iron overload curcumin group that received six doses of curcumin (1000 mg/kg BW by i.p.). In addition to six doses of iron dextran 1000 mg/kg(--1) by i.p., we studied the effects of curcumin on liver function enzymes (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]); antioxidant enzymes (malondialdehyde [MDA], total oxidant status [TOS], total antioxidant status [TAS]); hematological parameters (hemoglobin [Hb], hematocrit [Hct], red blood cells [RBC], white blood cells [WBC], mean corpus volume [MCV], mean corpuscular hemoglobin [MCH], mean corpuscular hemoglobin concentration [MCHC]); and iron parameters (serum iron profile, transferrin, total iron-binding capacity [TIBC], ferritin, and transferrin saturation [TS%]). Results: Curcumin caused a significant decrease in the Hct and Hb concentrations in Group III (P < 0.05). It also significantly reduced the serum levels of ALT (52.45 +/- 4.51 vs 89.58 +/- 4.65 U/L) and AST (148.03 +/- 6.47 vs 265.27 +/- 13.02 U/L) at the end of the study (P < 0.05). The TIBC, transferrin levels, and TS significantly decreased when the rats were administered curcumin serum iron (P < 0.05). The TAS level significantly increased in Group III in comparison to Group I (the control group) (P < 0.05). At the end of the study, curcumin significantly reduced the serum levels of TOS (12.03 +/- 2.8 vs 16.95 +/- 5.05 mmol H2O2/L) while the TAS (1.98 +/- 0.42 vs 1.06 +/- 0.33 mmol Trolox equiv./L) was increased. Conclusion: The findings of the present study suggest the therapeutic potential of curcumin against iron overload.
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    IN VITRO EFFECTS OF ANTIOXIDANT AND PROAPOPTOTIC ACTIVITIES OF THYMOQUINONE IRON COMPLEX
    (Dokuz Eylul Univ Inst Health Sciences, 2022) Ozbolat, Guluzar; Alizade, Ares
    Introduction: This study aimed to investigate the proapoptotic and antioxidant effects of the Thymoquinone (TQ) iron complex on the SW480 cell line. This study investigates the proapoptotic and antioxi-dant effects of the TQ iron complex on the SW480 cell line. Material and Methods: The SW480 cells were routinely cultured in a medium for 48 h. and incubated at 37 degrees C in a 5% CO2 in the air. After the incubation period, the cells were washed with buffer, and 100 ml of the denaturing lysis buffer per 0.5 was added to 2x10(7) cells for 15 min, and supernatants were tak-en. ELISA test was used to examine the expression and activity of GADD153, Wee1, cleaved Caspase-3, Bax, GRP78, and Bcl-2 proteins in SW480 cells. In this study, to measure activities of total antioxidant capacity (TAS), catalase (CAT), total oxidant capacity (TOS), and superox-ide dismutase (SOD) activities were investigated by the ELISA method in cell lines SW480 treated with the TQ iron complex. Results: ELISA test results indicated that the activities of apoptotic proteins Bax, Wee1 Caspase-3, GADD153, GRP78, and Bcl-2 in human SW480 cell lines were significantly increased in the 48-hour treatment. Conclusion: Our results of this study demonstrated that in untreated cultures, high TAS, SOD and CAT ac-tivities were found in SW480 cell lines than in control cell lines.
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    In vitro effects of iron chelation of curcumin Fe (III) complex
    (Cukurova Univ, Fac Medicine, 2019) Ozbolat, Guluzar; Yegani, Arash Alizadeh
    Purpose: The aim of this study was to investigate the cytotoxicity effect, iron chelator and antioxidant activities of iron (III) ions with curcumin ligand that may be used in the treatment of iron overload. Materials and Methods: The cytotoxic activities of the ligand and the complex were evaluated by the MTT assay. The SOD activity of the complex of curcumin was determined by using its ability to inhibit the reduction of NBT. The catalytic activity studies of Fe(III) complex in DMSO towards the disproportionation of hydrogen peroxide were also performed. Results: The IC50 values are found in 6.8 mu M catalase activity was measured. Where at a concentration of 2.0 mM, the activity was equivalent to 183.30 U/L. The complex shows a catalase activity. The complex showed minimal toxicity. IC50 values found 5.3 mg/ml. The observed cytotoxicity could be pursued to obtain a potential drug. The iron chelator effects were determined by Ferrozine reagent. Curcumin, the most active extract interfered with the formation of ferrous and ferrozine complex. It demonstrated strong chelating activities. The result showed that the complexes possess considerable SOD activity. This finding indicates that the iron complex is capable of removing free radicals. Conclusion: The study results revealed that the iron(III) complex of curcumin with an appropriate potential drug may act as a protector against oxidative stress. Therefore, all results suggest that curcumin may represent a new approach in the treatment of iron overload.
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    IN VITRO STUDIES ON THE PROTECTIVE EFFECT OF TANNIC ACID OF U87 CELLS INDUCED BY BETA-AMYLOID
    (Dokuz Eylul Univ Inst Health Sciences, 2021) Ozbolat, Guluzar; Alizade, Ares
    Background: While the prevalence of Alzheimer's disease continues to increase throughout the twentieth century, the cause and pathology of the disease are not well understood and scientists are seeking treatments for the disease. Tannic acid can be used effectively to treat Alzheimer's disease and seems to be one of the alternative therapeutic strategies in medicine. In this study, we aimed to investigate the effects of tannic acid on the U87 (human astrocytoma cell line) treated with amyloid-beta (A beta), which is the Alzheimer's disease (AD) model cell line. Materials and Methods: In the study; three groups were formed as the control group, the A beta group, and the A beta + tannic acid group obtained by adding tannic acid to the A beta group. Firstly, the cytotoxic potential of TA in U87 cells was investigated by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5 diphenyltetrazolium bromide) test. To determine the antioxidant status in the cell line treated with tannic acid, to examine the effects of total oxidant status (TOS), superoxide dismutase (SOD), total antioxidant status (TAS) and catalase (CAT) activities, were measured by the ELISA method. Results: In our study, the viability and proliferation of the cell decreased in U87 cells treated with amyloidB compared to the control group, but tannic acid increased cell viability and proliferation when compared with the group treated with amyloid-B. When compared to the control group, the TAS, SOD, and CAT levels were significantly decreased in the U87 cell line exposed to A beta; TOS levels were found to increase significantly. Conclusions: In in vitro experiments, we determined that tannic acid has a protective effect by increasing antioxidant parameters in the amyloid beta-induced cell line.
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    Investigation of apoptotic effects of D-pantothenic acid on PC-3 prostate cancer cells
    (Cukurova Univ, Fac Medicine, 2020) Alizade, Ares; Ozbolat, Guluzar
    Purpose: The anti-inflammatory and antioxidant properties of D-pantothenic acid have been demonstrated and the effects of dexpentanol on inflammatory pathways and apopototic pathways that trigger cell death are of interest. Apoptotic pathways are important in resistance to chemotherapeutics in cancer diseases and in cancer development. Therefore, we planned how treatment of PC-3 human prostate cancer cells with dexpanthenol will affect the levels and activities of apoptotic and inflammation mediators. For this purpose, human prostate cancer cell culture was performed. Materials and Methods: The human prostate cancer cells were treated with dexpentanaol then protein levels and activities of inflammatory and apoptotic pathway mediators such as gadd153, AIF, grp78, bax and bcl-2 in the cells were analyzed by ELISA. Results: The results of our study showed that, Dpantothenic acid did not statisticaly decreased the leves of bax, bcl-2 and grp78 protein expression in PC-3 prostate cancer cells. The effect of D-pantothenic acid on gadd153 and AIF proteins in PC-3 cells was increased but this increased level did not statisticaly significant. Conclusion: Recent studies have demonstrated the potential benefits of anti-inflammatory drugs. Our study showed that D-pantothenic acid had no significant effect on the growth of PC-3 cells and has no significant effect on intracellular apoptotic pathways.
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    Regulation of Nrf2 and Nrf2-related proteins by ganoderma lucidum in hepatocellular carcinoma
    (Springer, 2022) Aslaminabad, Ramin; Rahimianshahreza, Negin; Hosseini, Seyed Amirhossein; Armagan, Guliz; Khan, Ahmad Kashif; Ozbolat, Guluzar; Ahmed, Omar Saad
    Background HCC is among the most common cancer. Ganoderma lucidum (G.lucidum) has been essential in preventing and treating cancer. The Nrf2 signaling cascade is a cell protective mechanism against further damage, such as cancer development. This signaling pathway upregulates the cytoprotective genes and is vital in eliminating xenobiotics and reactive oxygen. This study aimed to show the potential cytotoxic activity of G. lucidum aqueous extract in HCC. Methods and results MTT assay was used to detect cell viability. Nrf2-related proteins were measured by western blotting, and the flow cytometry method assayed cell population in different cycle phases. Cell viability was 49% and 47% following G. lucidum extract at 100 mu g/ml at 24 and 48 h treatments, respectively. G. lucidum extract (aqueous, 100 or 50 mu g/ml) treatments for 24, 48, or 72 h were able to significantly change the cytoplasmic/nuclear amount of Nrf2 and HO-1, NQO1 protein levels. Moreover, at both concentrations, arrest of the G0/G1 cell cycle was stimulated in HCC. Conclusions The activation of the Nrf2 signaling pathways seems to be among the mechanisms underlining the protective and therapeutic action of G. lucidum against HCC.
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    Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand
    (Wiley, 2020) Ozbolat, Guluzar; Yegani, Arash Alizadeh
    Iron overload is a key target in drug development. This study aimed to investigate the coordination of Fe(III) ions with a curcumin-oxime ligand that may be used in the treatment of iron overload. The synthesis of the curcumin-oxime ligand and curcumin-oxime-Fe(III) complex was successfully made and characterized in its solid-state and solution-state using FT-IR, UV-Vis, elemental analysis, and H-1-NMR. However, in this study, we investigated the apoptotic effects of the curcumin-oxime Fe (III) complex on SW480. SW480 cells were exposed to 99.2% medium for 48 hours. After 48 hours, the incubation period, cells were harvested by centrifugation and washed in phosphate-buffered saline (PBS) and lysed in radio-immunoprecipitation assay (RIPA) buffer for 20 minutes and supernatants were taken and pellets were discarded. ELISA test was used to examine the expression, and activity of cleaved caspase-3, Bax, and Bcl-2 proteins in SW480 cells. ELISA test results indicated that the activities of apoptotic proteins Bax, caspase 3 and Bcl-2 in human SW480 cell lines significantly increased in 48 hours treatment. Also, the activity of Bcl-2 was observed to decrease significantly. Catalase activities of the complex were investigated. The findings showed that the complex has a catalase activity. The findings suggest that this type of complex may constitute a new and interesting basis for the future search of new and more potent drugs. The SOD activity of the result showed that the complexes possessed a considerable SOD activity with an IC50 value of 7.685 mu M. Also, when compared with the control, a complex increased the SOD levels (P < .05). Electrochemistry studies in the literature have shown that the Fe3+/Fe(2+)couple redox process occurs in low potential. This value is within the range of compounds that are expected to show superoxide dismutase activity. The I-pc/I(pa)shows that one electron transport takes place in the complex. Our results suggest that curcumin-oxime may represent a new approach in the treatment of iron overload.