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    Development of molecular imprinted nanosensor for determination of tobramycin in pharmaceuticals and foods
    (Elsevier Science Bv, 2014) Yola, Mehmet Lutfi; Uzun, Lokman; Ozaltin, Nuran; Denizli, Adil
    In this study, we developed quartz crystal microbalance (QCM) nanosensor for the real-time detection of tobramycin (TOB). Firstly, the modification of gold surface of QCM chip was performed by self-assembling monolayer formation of allyl mercaptane to introduce polymerizable double bonds on the chip surface. Then, TOB imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) [p(HEMA-MAGA)] film was generated on the gold surface. The nonmodified and TOB-imprinted p(HEMA-MAGA) surfaces were characterized by using atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, ellipsometry and contact angle measurements. The proposed method was validated according to the ICH guideline. The linearity range and the detection limit (S/N=3) were obtained as 1.7 x 10(-11)-1.5 x 10(-10) M and 5.7 x 10(-12) M, respectively. The developed method was applied to pharmaceuticals, and food samples such as chicken egg white and milk extract for the determination of TOB. In addition, association kinetics analysis and isotherm models were applied to the data to explain the adsorption process that took place. (C) 2013 Elsevier B.V. All rights reserved.
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    Öğe
    Molecular imprinted polypyrrole modified glassy carbon electrode for the determination of tobramycin
    (Pergamon-Elsevier Science Ltd, 2013) Gupta, Vinod Kumar; Yola, Mehmet Lutfi; Ozaltin, Nuran; Atar, Necip; Ustundag, Zafer; Uzun, Lokman
    Over the past two decades, molecular imprinted polymers have attracted a broad interest from scientists in sensor development. In the preparation of molecular imprinted polymers the desired molecule (template) induces the creation of specific recognition sites in the polymer. In this study, the glassy carbon electrode (GCE) based on molecularly imprinted polypyrrole (PPy) was fabricated for the determination of tobramycin (TOB). The developed electrode was prepared by incorporation of a template molecule (TOB) during the electropolymerization of pyrrole on GCE in aqueous solution using cyclic voltammetry (CV) method. The performance of the imprinted and non-imprinted electrodes was evaluated by square wave voltammetry (SWV). The effect of pH, monomer and template concentrations, electropolymerization cycles on the performance of the imprinted and non-imprinted electrodes was investigated and optimized. The non-modified and TOB-imprinted surfaces were characterized by using atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS) and CV. The linearity range of TOB was 5.0 x 10(-10)-1.0 x 10(-8) M with the detection limit of 1.4 x 10(-10) M. The developed nanosensor was applied successfully for the determination of TOB in egg and milk. (C) 2013 Elsevier Ltd. All rights reserved.