Yazar "Khaleghi, Fatemeh" seçeneğine göre listele

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    Fabrication of novel electrochemical sensor for determination of vitamin C in the presence of vitamin B9 in food and pharmaceutical samples
    (Elsevier Science Bv, 2016) Khaleghi, Fatemeh; Arab, Zahra; Gupta, Vinod Kumar; Ganjali, M. R.; Norouzi, Parviz; Atar, Necip; Yola, Mehmet L.
    Synthesis and application of NiO-multiwall carbon nanotube nanocomposite (NiO/MWCNTs) and 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4) in the carbon paste matrix as high sensitive sensors for voltammetric determination of vitamin C have been reported. The oxidation peak potential of vitamin C at the surface of the ([Bmim]BF4) NiO/MWCNT carbon paste electrode (NiO/MWCNTs/([Bmim]BF4/CPE) appeared at 440 mV, which was about 200 mV lower than the oxidation peak potential at the surface of the simple carbon paste electrode under a similar condition. Also, the oxidation peak current was increased for about 3.7 times at the surface of NiO/MWCNTs/([Bmim]BF4/CPE compared to carbon paste electrode. At an Optimum,condition (pH 7.0), the two peaks are separated ca. 0.44 and 0.85 V for vitamin C and vitamin B-9. This point shows that vitamin C can be determined in the presence of vitamin B-9. The linear response range and detection limit were found to be 0.1-1000 mu M and 0.06 mu M, respectively. The proposed sensor was successfully applied, for the determination of vitamin C in food and drug samples. (C) 2016 Elsevier B.V. All rights reserved.
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    Silver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines
    (Tehran University of Medical Sciences, 2022) Darvish, Soodabeh; Kahrizi, Mohammad Saeed; Özbolat, Gülüzar; Khaleghi, Fatemeh; Mortezania, Zohreh; Sakhaei, Delaram
    Objective(s): A study was performed on the possibility of synthesizing silver nanoparticles (Ag-NPs) from Ducrosia Anethifolia Aqueous Extract for treating breast cancer MCF-7 and MDA-MB-231 cell lines. Methods: The application of Ducrosia Anethifolia Aqueous Extract caused a reduction in silver ions and facilitated the production of silver nanoparticles. Different analyzing procedures were exerted to evaluate the qualities of synthesized NPs by Ducrosia Anethifolia Aqueous Extract including UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). In the following, subsequent to assessing the viability of MCF- 7 and MDA-MB-231 cell lines, their exposure to AgNPs was evaluated by the usage of MTT assay. Moreover, the Bax, Bad, Bcl-2, and c-FLIP expression levels of treated cells were examined by real-time PCR analysis. Results: The observance of an absorption peak at 460 nm through the obtained data from a spectrophotometer affirmed the production of silver NPs. The images of electron microscope displayed the spherical shape of NPs in an average size of around 9.41 nm. Moreover, this product caused a robust reduction in the viability of both MCF-7 and MDA-MB-231 cell lines that was mainly pointed out by the improved expressions of Bax and Bad gene, while minimizing the gene expressions of Bcl-2 and c-FLIP. Conclusions: This experiment approved the applicability of Ducrosia Anethifolia Aqueous Extract for performing the green synthesis of NPs. In addition, the synthesized NPs exhibited anti-tumor effects on the applied breast cancer cell lines through the up-regulation of pro-apoptotic proteins concomitant along with the down-regulation of anti-apoptotic proteins expression. © Semina:Ciencias Agrarias.All right reserved.