Yazar "Kalindemirtas, Ferdane Danisman" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    Iron(III) complexes based on tetradentate thiosemicarbazones: Synthesis, characterization, radical scavenging activity and in vitro cytotoxicity on K562, P3HR1 and JURKAT cells
    (Wiley, 2021) Kalindemirtas, Ferdane Danisman; Kaya, Busra; Bener, Mustafa; Sahin, Onur; Kuruca, Serap Erdem; Demirci, Tulay Bal; Ulkuseven, Bahri
    Nine iron(III) complexes, [Fe(L)Cl], were synthesized starting from S-alkyl-thiosemicarbazones and some substituted aldehydes. The L ligands formed by template condensation are N2O2-chelating structures and named N-1-acetylacetone-N-4-R-salicylidene-S-alkyl-thiosemicarbazidato (L2-) where alkyl = methyl, propyl, or allyl and R = 3-methoxy, 4-methoxy, or 3,5-dichloro. The complexes were characterized using elemental analysis, IR, and ESI-MS. X-ray diffraction analysis of complex Fe8 (as a representative sample) indicated a square pyramid environment of the iron ion. The cytotoxicity performances of the complexes were determined using chronic myelogenous leukemia (K562), Burkitt's lymphoma (P3HR1), and T-cell leukemia (JURKAT) cell lines. For comparison, the noncancerous cell lines, human umbilical vein endothelial (HUVEC) and diploid fibroblast (3T3), and imatinib as positive control were included in the study. MTT results revealed that complexes Fe2, Fe5, Fe7, and Fe8 with methoxy (OCH3) substituent have remarkable cytotoxic effects on K562 and P3HR1 cells at relatively low concentrations in the ranges of 4.81-14.05 and 5.61-11.98 mu M, respectively. The radical scavenging activities of the complexes were measured for DPPH, superoxide anion (O-2(center dot-)), hydroxyl (center dot OH) radicals, and hydrogen peroxide (H2O2). Complexes Fe2, Fe3, Fe5, and Fe8, which exhibited selective cytotoxicity, were able to compete also with vitamin E in terms of ROS scavenging activities.
  • [ X ]
    Öğe
    New oxovanadium(IV) complexes overcame drug resistance and increased in vitro cytotoxicity by an apoptotic pathway in breast cancer cells
    (Elsevier Ireland Ltd, 2022) Kalindemirtas, Ferdane Danisman; Kaya, Buesra; Sert, Esra; Sahin, Onur; Kuruca, Serap Erdem; Ulkuseven, Bahri
    In order to examine the anticancer potential of oxovanadium(IV) complexes with thiosemicarbazone, two new complexes were prepared starting from 2-thenoyltrifluoroacetone-S-methylthiosemicarbazone. The complexes with tetradentate thiosemicarbazone ligand were characterized by elemental analysis, IR, ESI MS, and single crystal X-ray diffraction analysis. Cytotoxicity on breast cancer cells, MDA-MB-231 and MCF-7, was determined by MTT assay. Cisplatin was positive control and the results were compared with those of the normal cells, HUVEC and 3T3. The complexes exhibited greater activity on cancer cells than cisplatin, but they were cytotoxic at several times higher concentrations in the healthy cells. In our study, the presence of thiophene and fluoro groups in the oxovanadium(IV) complexes with thiosemicarbazone increased greatly the cytotoxic activity of the complexes on breast cancer cells. Moreover, the complexes induced apoptosis-mediated cell death and also reduced the expression of MDR-1 or P-glycoprotein and ABCG2. As a result, the findings indicated that the complexes have selective cytotoxicity on breast cancer cells and can overcome multidrug resistance. These properties of the complexes make it possible to be a potential anticancer drug candidate for breast cancer treatment.
  • [ X ]
    Öğe
    Oxovanadium(IV) complexes with tetradentate thiosemicarbazones. Synthesis, characterization, anticancer enzyme inhibition and in vitro cytotoxicity on breast cancer cells
    (Pergamon-Elsevier Science Ltd, 2021) Ertik, Onur; Kalindemirtas, Ferdane Danisman; Kaya, Busra; Yanardag, Refiye; Kuruca, Serap Erdem; Sahin, Onur; Ulkuseven, Bahri
    Five oxovanadium(IV) complexes were synthesized using acetyl-and benzoylacetone-S-alkyl-thiosemicarbazones (alkyl=methyl, ethyl, propyl or butyl) and salicylaldehyde. Structural characterization was performed by element analysis, infrared, mass and electronic spectra, and also single crystal X-ray crystallography for one sample. The purity of all the complexes was verified with Miller indices (hkl) observed and calculated angles, 2h values and crystal sizes obtained from powder XRD diffraction. The in vitro cytotoxic activity was determined for the MCF-7, MDA-MB-231 and 3T3 cell lines with an MTT assay for 72 h. The complexes showed cytotoxicity against MCF-7, MDA-MB-231 and 3T3 cells at concentrations of 73- 148, 75-224 and 112-176 mu M, respectively. All the complexes had better cytotoxicity than the positive control, fluorouracil (5-FU). The complex containing the S-propyl group was the most effective complex, with the concentrations of 72.9 (for MCF-7) and 75.5 (for MDA-MB-231) mu M. Furthermore, the ability of the complexes to inhibit elastase, xanthine oxidase and neuraminidase enzymes and their role in cell death was studied. All the complexes inhibited the elastase and xanthine oxidase enzymes at higher concentrations than the IC50 values found for the cytotoxicity. However, the IC50 values for the inhibition of neuraminidase by the complexes bearing ethyl and propyl groups are better than those of quercetin and are close to the values found for the cytotoxicity. (C) 2021 Elsevier Ltd. All rights reserved.