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    Öğe
    Novel Schiff base-Pt(ii) complexes: inhibition of Aβ aggregation via histidine interaction
    (Royal Soc Chemistry, 2026) Irisli, Sevil; Dogan, Umut; Gunnaz, Salih; Yurt, Fatma; Sahin, Onur
    In this study, three Schiff base ligands (L1-L3) and their novel platinum(ii) complexes (I-III) were synthesized and characterized using FT-IR, NMR, and elemental analysis. The crystal structure of complex I was determined by X-ray crystallography, while the lipophilicity of the complexes was evaluated by UV-Vis spectroscopy. The ability of the compounds to inhibit A beta aggregation was assessed using the SH-SY5Y human neuroblastoma cell line. Due to its high cytotoxicity, comparable to that of cisplatin, complex II was excluded from further biological investigations. The kinetics of A beta aggregation inhibition were examined fluorometrically using Thioflavin-T, and the binding interactions of the complexes with the A beta 1-42 sequence were elucidated through studies of their interactions with l-histidine using 1H-NMR and LC/QTOF/MS analyses. The results demonstrate that complexes I and III significantly suppress A beta fibril formation, with IC50 values of 50 mu M and 25 mu M, respectively. The enhanced biological activity is attributed to the strong electron-donating properties of the ligand substituents. Overall, these findings reveal that the synthesized complexes effectively inhibit A beta amyloid aggregation and promote cell viability.
  • [ X ]
    Öğe
    The Amyloid Beta Aggregation Inhibition Properties of New Pt (II) Complexes and Their Potential in Alzheimer's Treatment
    (Wiley, 2025) Gunnaz, Salih; Sahin, Onur; Irisli, Sevil
    In this study, N-phenylortho-phenylenediamine and N-phenyl ethylenediamine were utilized as amines, and two novel ligands, benzimidazole (LI) and Schiff base (LII), were synthesized through condensation reactions with 3-methoxy-4-benzyloxybenzaldehyde. Furthermore, using these same amines, LIII (benzimidazole) with 3-phenoxybenzaldehyde and LIV (Schiff base) with 4-benzyloxybenzaldehyde were synthesized in accordance with the literature. Then, Pt (II) complexes (Ia, Ib, II, III, and IV) were prepared with these ligands, and it was determined that Ia and Ib are geometric isomers. The ligands and complexes were characterized using 1H-NMR, 13C-NMR, FT-IR, and elemental analysis methods. The cytotoxic effects of these complexes were evaluated on SH-SY5Y neuronal cells. Low-toxicity complexes Ia, II, and IV were then tested for their ability to inhibit amyloid beta (A beta 1-42) aggregation. Thioflavin T fluorescence analysis, AFM imaging, and MALDI-TOF mass spectrometry demonstrated that these complexes, particularly at a 1:1 complex-to-amyloid molar ratio, effectively inhibited A beta aggregation. These findings suggest that Pt (II) complexes containing Schiff base and benzimidazole ligands may be effective against Alzheimer's disease by alleviating A beta toxicity.