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    The response of meiobenthinc sediment-dwelling nematodes to pyrene: Results from open microcosms, toxicokinetics and in silico molecular interactions
    (Pergamon-Elsevier Science Ltd, 2022) Hedfi, Amor; Allouche, Mohamed; Hoineb, Faten; Ben Ali, Manel; Harrath, Abdel Halim; Albeshr, Mohammed Fahad; Mahmoudi, Ezzeddine
    An experiment conducted with 'open microcosm' made by connected sedimentary compartments with different qualities was for 30 days to evaluate the migratory behaviour of nematodes as induced by exposure to pyrene. The nematofauna was collected along with sediment from a reference site in Bizerte lagoon. Following one week acclimatization period, the populated sediments were topped with azoic sediments, with a pyrene concentration of 150 mu g kg-1. The concentration of pyrene from sediments was measured on a weekly basis. A stable status of nematode assemblages was reached between the upper and lower compartments in each microcosm, whatever the treatment type. An upward exploratory phase was observed during the first two weeks within the assemblages, possibly induced by the repellent chemodetection of pyrene. This observation was confirmed by the toxicokinetic properties and molecular interactions of pyrene with the germ-line development Protein 3 and sexdetermining protein of Caenorhabditis elegans as nematode model.