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    Acute effects of combined and isolated caffeine and theanine supplementation on physical and cognitive performance in competitive athletes: a randomized, double-blind, placebo-controlled crossover study
    (Frontiers Media Sa, 2026) Tuncer, Selin Yildirim; Ozdenk, Serhat; Yildirim, Ulas Can; Erkan, Dilara; Sari, Cengizhan; Gundem, Mehmet Can; Sar, Halit
    Introduction Modern athletic performance is driven not only by physical capacity but also by rapid decision-making, attentional control, and visuomotor coordination. Evidence regarding the acute effects of caffeine (CAF), L-theanine (TEA), and their combination remains inconsistent, particularly with respect to their combined influence on physical and cognitive performance in athletic populations. This study examined the acute effects of isolated and combined CAF and TEA supplementation on maximal strength, intermittent aerobic endurance, and eye-hand coordination in competitive athletes. It was hypothesized that the combined ingestion of CAF and TEA would differentially affect physical performance and eye-hand coordination outcomes compared with isolated CAF or TEA intake.Methods Twenty trained athletes completed four randomized, double-blind, placebo-controlled crossover conditions: CAF (3 mgkg-1), TEA (200 mg), CAF+TEA (COM), and placebo (CON), with >= 72 h washout. Outcomes included isometric leg, back, and handgrip strength; Yo-Yo Intermittent Endurance Test Level 1 performance; and visuomotor coordination (CogniFit (R)). Data were analyzed using repeated-measures ANOVA with Bonferroni corrections (alpha = 0.05).Results Condition effects were found for leg strength (p = 0.004, eta p2 = 0.24) and back strength (p = 0.008, eta p2 = 0.19). In the COM condition, no additional or synergistic effect on maximal strength was observed. Additionally, no significant difference was found between the conditions in aerobic endurance, maximum isometric handgrip strength, and hand-eye coordination results. Caffeine ingestion did not differ from placebo for any strength outcome under the present conditions. Finally, acute ingestion of TEA was associated with reduced maximal isometric leg and back strength compared with CAF and CON.Discussion Findings challenge the prevailing assumption of CAF + TEA synergy and underscore the need for task-specific interpretation of co-supplementation strategies. Future studies should evaluate dose-response interactions, habitual caffeine intake, sex-specific responses, and broader cognitive domains beyond visuomotor control. The randomized controlled trial was registered at ClinicalTrials.gov, under the registration number NCT07268573.Clinical Trial Registration ClinicalTrials.gov, NCT07268573.
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    Biochemical Effects of Long-Term Exercise on Oxidative Stress and Antioxidant Markers in Adolescent Female Athletes
    (Mdpi, 2025) Bilici, Omer Faruk; Erkan, Dilara; Alexe, Dan Iulian; Tohanean, Dragos Ioan; Demir, Canan; Alexe, Cristina Ioana; Voiculescu, Virgil Ene
    Background: Adolescence is a critical period where exercise-induced oxidative stress is modulated by both training adaptations and hormonal changes, particularly the antioxidant effects of estrogen in females. However, data on how adolescent female athletes respond to long-term exercise remain limited. The aim of this study was to examine oxidative stress levels and some antioxidant defense parameters in adolescent female athletes who train regularly. Methods: The study included 20 adolescent female basketball players (16.65 +/- 0.67 years; 165.50 +/- 0.06 cm; 59.75 +/- 5.50 kg) with at least three years of training experience and 20 non-athlete adolescent female participants (16.80 +/- 0.69 years; 159.95 +/- 0.04 cm; 60.15 +/- 4.23 kg). Malondialdehyde (MDA), glutathione (GSH), and catalase (CAT) levels were analyzed by a spectrophotometric method using a UV/VIS spectrophotometer in blood samples taken from all participants, and the data were compared between the groups. Results: The results showed that MDA levels were significantly lower in the athlete group (p < 0.01; d = 4.78). In addition, CAT activity was significantly higher in athletes compared to non-athletes (p < 0.01; d = 7.81). However, no significant difference was observed in GSH levels between the groups (p > 0.05; d = 0.15). A strong negative correlation was found between MDA and CAT (r = -0.900). Conclusions: These findings suggest that prolonged exercise reduces oxidative stress and enhances catalase-mediated antioxidant defense in adolescent women. Increased CAT activity and decreased MDA levels support this effect, while stable GSH levels point to the role of compensatory mechanisms.
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    Comparative effects of caffeine and paraxanthine on rowing performance and sleep quality: a randomized crossover study
    (Taylor & Francis Inc, 2026) Bingol Diedhiou, Azize; Yildirim, Ulas Can; Ozdenk, Serhat; Erkan, Dilara; Karakulak, Izzet; Yildirim Tuncer, Selin; Turgut, Murat
    BackgroundAlthough caffeine is widely used in athletes due to its ergogenic effects, the effects of its main metabolite, paraxanthine, on performance and sleep have not been adequately investigated.PurposeThis study aimed to comparatively investigate the effects of caffeine and its main metabolite paraxanthine on rowing performance and sleep quality.MethodsThe study was designed as a randomized, double-blind, crossover study and included 14 male university-level rowers (21.6 +/- 1.9 age; 2.2 +/- 1 years of rowing experience). The participants participated in 2000-m rowing ergometer time trials under four different supplementation conditions (caffeine + paraxanthine, caffeine + placebo, paraxanthine + placebo, and placebo with 200 mg each). Performance data (completion time, mean power, and heart rate), sleep quality, and daytime sleepiness were assessed by subjective scales. The data were analyzed by repeated-measures analysis of variance (ANOVA) and Bonferroni post-hoc tests.ResultsCompared with the placebo, the combined caffeine + paraxanthine condition was associated with faster 2000-m performance and higher mean power output (p = 0.044; Cohen's d = 0.30). Caffeine alone and paraxanthine alone did not show clear evidence of performance improvement in this sample, although estimates favored both conditions versus placebo. Conditions containing caffeine were associated with poorer subjective sleep quality, whereas paraxanthine alone showed more favorable sleep-related outcomes.ConclusionThese results indicate that the combined ingestion of caffeine and paraxanthine elicited the most pronounced performance benefits, while paraxanthine alone did not demonstrate clear standalone ergogenic efficacy at the administered dose. However, paraxanthine was associated with better subjective sleep outcomes compared to caffeine, suggesting that its potential value may be related more to tolerability rather than superior performance enhancement, particularly for athletes training in the evening. Study limitations, including the small sample size and lack of objective sleep measures, should be considered when interpreting the results.Practical applicationsParaxanthine may represent a practical option for athletes who prioritize sleep quality or experience sensitivity to caffeine-related sleep disturbances, although further dose-response studies are required to clarify its ergogenic potential.
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    The effect of low dose caffeine powder supplementation on serve speed, spike speed, and speed-endurance in elite sitting volleyball players: a randomized double-blind crossover study
    (Bmc, 2025) Diedhiou, Azize Bingol; Erkan, Dilara; Guler, Melek; Sar, Halit; Karakulak, Izzet; Eyuboglu, Ender; Gundem, Mehmet Can
    BackgroundSitting volleyball relies heavily on upper-body strength and anaerobic capacity. Serve, spike, and speed-endurance are decisive skills, yet the ergogenic potential of low-dose caffeine in this Paralympic sport remains unclear.PurposeTo examine the acute effects of low-dose caffeine (3 mg/kg) supplementation on serve speed, spike speed, and speed-endurance in elite sitting volleyball players.MethodsUsing a randomized, double-blind, crossover design, 13 elite male athletes from the Turkish National Sitting Volleyball Team completed serve speed, spike speed, and speed-endurance tests under caffeine (CAF) and placebo (PLA) conditions.ResultsCaffeine intake produced a moderate improvement in serve speed (p = 0.028, d = 0.460); however, this effect did not remain statistically significant after Bonferroni correction (adjusted p = 0.084). No significant effects were observed for spike speed (p = 0.547, d = 0.166) or speed-endurance performance (p = 0.709, d = 0.111). Perceived exertion during the speed-endurance test was similarly high in both conditions.ConclusionsLow-dose caffeine may offer a trend toward improved serve performance, but the effect was not robust after statistical adjustment, and no benefits were observed for spike speed or speed-endurance. These findings highlight that caffeine's ergogenic effects are context-dependent and shaped by task complexity and sport-specific motor demands. Further research with larger and more diverse samples, genotype-based subgroups, and varied dosing strategies is warranted to clarify caffeine's role in adaptive sports.Trial registrationThe randomized controlled trial was retrospectively registered on 21/06/2025 at ClinicalTrials.gov, under the registration number NCT07056231.
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    Varying doses of evening caffeine ingestion have different effects on rowing ergometer performance, sleep quality, and wakefulness scores
    (Frontiers Media Sa, 2025) Karakulak, Izzet; Yildirim, Ulas Can; Erkan, Dilara; Karayigit, Raci; Eyuboglu, Ender; Diedhiou, Azize Bingol; Gundem, Mehmet Can
    Introduction: This study investigated the dose-dependent effects of evening caffeine ingestion on rowing performance, sleep quality, and daytime sleepiness in trained male rowers. Methods: Using a double-blind, randomized, crossover design, 13 university-level rowers (mean age = 22.07 +/- 2.21 years; mean body mass = 77.66 +/- 6.45 kg) completed four 2,000 m time-trial sessions between 19:00 and 20:00 h under placebo (PLA), low-dose capsule caffeine (3 mg/kg, LDC), moderate-dose capsule caffeine (6 mg/kg, MDC), and high-dose capsule caffeine (9 mg/kg, HDC) conditions. Performance metrics, heart rate, and subjective sleep assessments were collected. Rowing performance was assessed by a standard 2,000 m rowing ergometer (Concept II, United States) time trial. Sleep quality was measured with a numerical rating scale in the morning after each trial, and daytime sleepiness was measured with the Karolinska Sleepiness Scale. Results: Results indicated significantly improved rowing times and power output with HDC and MDC compared to PLA (p < 0.05), with HDC yielding the most notable enhancements (d = 0.40-0.41). However, these ergogenic benefits were accompanied by significantly impaired sleep quality and elevated daytime sleepiness in both HDC and MDC groups (p < 0.01; d = 1.3-1.5). Notably, adverse effects such as headache, insomnia, and gastrointestinal discomfort were predominantly reported in the HDC condition (p < 0.05). Although LDC offered mild performance improvements with minimal sleep disruption, only the high dose condition exhibited large physiological and perceptual trade-offs. Discussion: These findings indicate a clear dose-response relationship, wherein higher evening caffeine intake improves performance but has detrimental effects on sleep and recovery markers. Coaches and athletes should carefully balance caffeine dosing against potential recovery costs, especially in evening training or competition contexts.