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    Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached L-glycine
    (Wiley, 2017) Donmez, Soner; Arslan, Fatma; Sari, Nursen; Ozkan, Elvan Hasanoglu; Arslan, Halit
    In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl) polystyrene (FMPS) with L-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 degrees C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 x 10(-4) to 1.0 x 10(-2) M, R-2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.