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Öğe Composite Cryogels Having Functional Hydrophobic Groups Preparation, Characterization and Use in DNA Adsorption(12th Eurasia Conference on Chemical Sciences, 2012) Çorman, Mehmet Emin; Bereli, Nilay; Özkara, Serpil; Uzun, Lokman; Denizli, Adil[Abstract Not Available]Öğe Development of molecular imprinted nanosensor for determination of tobramycin in pharmaceuticals and foods(Elsevier Science Bv, 2014) Yola, Mehmet Lutfi; Uzun, Lokman; Ozaltin, Nuran; Denizli, AdilIn this study, we developed quartz crystal microbalance (QCM) nanosensor for the real-time detection of tobramycin (TOB). Firstly, the modification of gold surface of QCM chip was performed by self-assembling monolayer formation of allyl mercaptane to introduce polymerizable double bonds on the chip surface. Then, TOB imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) [p(HEMA-MAGA)] film was generated on the gold surface. The nonmodified and TOB-imprinted p(HEMA-MAGA) surfaces were characterized by using atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, ellipsometry and contact angle measurements. The proposed method was validated according to the ICH guideline. The linearity range and the detection limit (S/N=3) were obtained as 1.7 x 10(-11)-1.5 x 10(-10) M and 5.7 x 10(-12) M, respectively. The developed method was applied to pharmaceuticals, and food samples such as chicken egg white and milk extract for the determination of TOB. In addition, association kinetics analysis and isotherm models were applied to the data to explain the adsorption process that took place. (C) 2013 Elsevier B.V. All rights reserved.Öğe İnsan Sağlığı için Toksik ve Kanserojen Etkiler Olusturan Benzo[a]piren’in Tayini.(18. Biyomedikal Mühendisligi Ulusal Toplantısı, 2014) Çorman, Mehmet Emin; Armutçu, Canan; Uzun, Lokman; Denizli, AdilSunulan çalışma kapsamında, çevre sularında eser miktarlarda bulunan benzo[a]piren (BaP)’in tayini amaçlanmıştır. Çalışmanın ilk aşamasında BaP baskılanmış poli(2-hidroksietil metakrilat-N-metakriloil-Lfenilalanin) monolitik polimerler, yığın polimerizasyonu yöntemi ile sentezlenmiştir. Elde edilen monolitik polimerler, öğütülerek eş boyutlu partiküller elde edilmiştir. Öğütülen polimerler elenerek 64-71 ?m boyut aralığındaki partiküller ayrılmış ve daha sonraki işlemlerde kullanılmıştır. BaP baskılanmış kompozit kartuşlar ise BaP baskılanmış partiküllerin varlığında, 2-hidroksietil metakrilat (HEMA) ve metilen bisakrilamidin (MBAAm), -12°C’da 24 saatlik yarı-donmuş koşullar altındaki polimerizasyonu ile üretilmiştir. Kartuşlar, şişme testi, taramalı elektron mikroskobu (SEM), elementel analiz ve Fourier dönüşümlü infrared spektroskopisi (FTIR) yöntemleri ile karakterize edilmiştir. Hazırlanan kompozit kartuşlar, sulu çözeltilerden BaP ön-deriştirme işlemlerinde kullanılmış ve ön-deriştirme koşulları optimize edilmiştir. Optimizasyon çalışmaları kapsamında; BaP derişimi, pH, santrifüj hızı, sıcaklık, iyonik şiddet, monomer oranı ve partikül miktarının etkileri incelenmiştir. Kompozit kartuşların diğer polisiklik aromatik hidrokarbon (PAH) türlerine karşı seçicilikleri, benzo[b]floranten (BbF), benzo[k]floranten (BkF), indeno[1,2,3-cd]piren (IcdP) ve 1-naftol varlığında gerçekleştirilen seçicilik çalışmaları ile belirlenmiştir.Öğe Lysine Imprinted Nanoparticles for Antibody Biorecogniton(2-nd International Conference Nanomaterials: Applications & Properties- Vol. 1 No 2, 02NNBM21(2pp), 2012) Çorman, Mehmet Emin; Armutçu, Canan; Uzun, Lokman; Say, Rıdvan; Denizli, AdilThe aim of this study was to prepare L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles which can be used for the adsorption of IgG from aqueous solutions. L-lysine was complexed with MAAsp and Llysine- imprinted poly(HEMA-MAAsp) nanoparticles were synthesized by miniemulsion polymerization. Also, non-imprinted nanoparticles were synthesized without L-lysine for control purpose. L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles were characterized by means of elemental analysis, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM).Öğe Molecularly imprinted cryogel cartridges for the specific filtration and rapid separation of interferon alpha(Royal Soc Chemistry, 2015) Corman, Mehmet Emin; Armutcu, Canan; Ozkara, Serpil; Uzun, Lokman; Denizli, AdilIn this study, we synthesised specific filtration cartridges with selective recognition sites for target molecules and used them to separate interferon alpha-2b from aqueous solutions. We combined molecular imprinting technology with cryogel to achieve specific and rapid filtration of interferon alpha-2b through the macroporous structure of a cryogel network. Recombinant interferon alpha-2b-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan) P(HEMATrp)/alpha-2bIFN cryogels were synthesised via free-radical bulk polymerisation under partially frozen conditions. The interferon alpha-2b filtration conditions were subsequently optimised with respect to factors such as pH, initial concentration, temperature, centrifugation speed, salt concentration and type and the amount of precomplex incorporated. Selectivity experiments were conducted in respect to isoelectric point as well as size of the competitor proteins under both uncompetitive and competitive conditions. The relative selectivity coefficients of the specific filtration cartridge in respect to isoelectronic points for interferon/ IgG, interferon/HSA and interferon/insulin pairs were 3.72, 7.10 and 10.68 times greater than the coefficient of a non-imprinted [P(HEMATrp)] filtration cartridge, respectively. Similarly, the relative selectivity coefficients of the specific filtration cartridge in respect to competitor size for interferon/ lysozyme, interferon/myoglobin and interferon/carbonic anhydrase pairs were calculated as 7.58, 10.40 and 11.68 under uncompetitive conditions whereas those values under competitive conditions were calculated as 1.08, 1.05 and 1.34, respectively. The results indicated that specific filtration cartridges developed could repeatedly adsorb interferon alpha-2b with a short separation time without any significant decrease in the adsorption capacity even if competitive conditions were conducted.Öğe Production of L-Histidine Imprinted Fluorescent Nanoparticles(2nd International Conference on Bio-Sensing Technology, 2011) Çorman, Mehmet Emin; Üzek, Recep; Güngüneş, Hakan; Şenel, Serap; Uzun, Lokman; Say, Rıdvan; Denizli, Adil[Abstract Not Available]Öğe Rapid, efficient and selective preconcentration of benzo[a]pyrene (BaP) by molecularly imprinted composite cartridge and HPLC(Elsevier, 2017) Corman, Mehmet Emin; Armutcu, Canan; Uzun, Lokman; Denizli, AdilIn this study, cryogel-based molecularly imprinted composite cartridges were designed for the rapid, efficient, and selective preconcentration of benzo[a]pyrene (BaP) from water samples. First, a BaP-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-phenylalanine) composite cartridge was synthesized under semi-frozen conditions and characterized by scanning electron microscopy, elemental analysis, Fourier transform infrared spectroscopy, and swelling tests. After the optimization of preconcentration parameters, i.e., pH and initial BaP concentration, the selectivity and preconcentration efficiency, and reusability of these cartridges were also evaluated. In selectivity experiments, BaP imprinted composite cartridge exhibited binding capacities 3.09, 9.52, 8.87, and 8.77-fold higher than that of the non-imprinted composite cartridge in the presence of competitors, such as benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), indeno[1,2,3-cd]pyrene (lcdP), and 1-naphthol, respectively. The method detection limit (MDL), relative standard deviation (RSD) and preconcentration efficiency (PE) of the synthesized composite cartridge were calculated as 24.86 mu g/L, 1.60%, and 349,6%, respectively. (C) 2016 Elsevier B.V. All rights reserved.Öğe Reversible and easy post-crosslinking method for developing a surface ion-imprinted hypercrosslinked monolith for specific Cd(II) ion removal from aqueous solutions(Royal Soc Chemistry, 2016) Corman, Mehmet Emin; Armutcu, Canan; Uzun, Lokman; Say, Ridvan; Denizli, AdilIn this study, a new surface imprinting technique for preparing a hypercrosslinked monolith to remove Cd(II) ions out from aqueous solutions was proposed. The monoliths were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermal gravimetrical analysis, surface area measurements and elemental analysis. The reversible nature of the hypercrosslinking process was proved by repeated crosslinking and denaturation cycles by using ferric ions as an oxidant and urea as a reductant, respectively meanwhile performing surface area measurements for both situations to demonstrate the variation in the surface porosity. The multipoint BET surface areas of poly(HEMA), surface ion imprinted (Cd-SII-HM) and non-imprinted (NI-HM) monoliths were determined as 269.1 m(2) g(-1), 79.1 m(2) g(-1), and 67.4 m(2) g(-1), respectively. By breaking hypercrosslinks, the micropore volume decreased from 39.7 mm3 g-1 to 11.8 mm(3) g(-1) while the cumulative pore volume decreased from 30.7 mm(3) g(-1) to 9.1 mm(3) g(-1) during urea treatment. At the first step, the affecting factors such as initial Cd(II) ion concentrations, pH and adsorption time were optimized. Then, the selectivity of the Cd-SII-HM for Cd(II) against other metal ions was evaluated not only from singular solutions but also from triple and quadruple solutions, which included Pb(II), Zn(II) and Hg(II) ions as competitors. The relative selectivity coefficients were calculated as 3.28, 15.61 and 58.55 for Cd(II)/Zn(II), Cd(II)/Pb(II) and Cd(II)/Hg(II) pairs. The results obtained indicated that the developed reversible and easy post-crosslinking method is quite applicable for producing the surface ion-imprinted polymers with high selectivity for the template ions [Cd(II)] regarding the potential competitor ions [Zn(II), Pb(II) and Hg(II)].Öğe Self-oriented nanoparticles for site-selective immunoglobulin G recognition via epitope imprinting approach(Elsevier, 2014) Corman, Mehmet Emin; Armutcu, Canan; Uzun, Lokman; Say, Ridvan; Denizli, AdilMolecular imprinting is a polymerization technique that provides synthetic analogs for template molecules. Molecularly imprinted polymers (MIPs) have gained much attention due to their unique properties such as selectivity and specificity for target molecules. In this study, we focused on the development of polymeric materials with molecular recognition ability, so molecular imprinting was combined with miniemulsion polymerization to synthesize self-orienting nanoparticles through the use of an epitope imprinting approach. Thus, L-lysine imprinted nanoparticles (LMIP) were synthesized via miniemulsion polymerization technique. Immunoglobulin G (IgG) was then bound to the cavities that specifically formed for L-lysine molecules that are typically found at the C-terminus of the Fc region of antibody molecules. The resulting nanoparticles makes it possible to minimize the nonspecific interaction between monomer and template molecules. In addition, the orientation of the entire IgG molecule was controlled, and random imprinting of the IgG was prevented. The optimum conditions were determined for IgG recognition using the imprinted nanoparticles. The selectivity of the nanoparticles against IgG molecules was also evaluated using albumin and hemoglobin as competitor molecules. In order to show the self-orientation capability of imprinted nanoparticles, human serum albumin (HSA) adsorption onto both the plain nanoparticles and immobilized nanoparticles by anti-human serum albumin antibody (anti-HSA antibody) was also carried out. Due to anti-HSA antibody immobilization on the imprinted nanoparticles, the adsorption capability of nanoparticles against HSA molecules vigorously enhanced. It is proved that the oriented immobilization of antibodies was appropriately succeeded. (C) 2014 Elsevier B.V. All rights reserved.
