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Öğe An Electrophoretic Taxonomic Study on Sarcoplasmic Proteins of Some Balithorid and Cobitid Fishes Habitated in Kars Stream, Turkey(Kafkas Üniversitesi Fen ve Mühendislik Bilimleri Dergisi,, 2008) Yılmaz, Muhittin; Ildes, Erhan; Alaş, Ali; Koç, EvrenIn this study, sarcoplasmic proteins of Orthrias tigris, Orthrias angorae bureschi, Orthrias panthera and Cobitis taenia were analyzed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In the electrophoregram, 13 sarcoplasmic protein bands were obtained from Orthrias angorae bureschi and Orthrias panthera. Nevertheless, 5 protein bands of these fishes were mutually calculated and similarity coefficient (SC) was found as 0.384. Sarcoplasmic protein bands of Orthrias tigris and Cobitis taenia were 8 and 12, respectively. 2 sarcoplasmic protein bands of Orthrias tigris and Cobitis taenia were found to be similar and similarity coefficient (SC) was 0.166. However, 2 sarcoplasmic protein bands of Cobitis taenia and Orthrias angorae bureschi were similar and similarity coefficient (SC) was found as 0.153. All the sarcoplasmic protein bands of Cobitis taenia and Orthrias panthera were observed to be different. On the other hand, 1 sarcoplasmic protein band of Orthrias angorae bureschi and Orthrias tigris was found to be similar and similarity coefficient (SC) was found as 0.076. 3 sarcoplasmic protein bands of Orthrias tigris and Orthrias panthera were found to be similar and similarity coefficient (SC) was found as 0.230. Similarities and differences of Orthrias tigris, Orthrias angorae bureschi, Orthrias panthera and Cobitis taenia which highly resemble each other morphologically in term of sarcoplasmic protein bands were electrophoretically found out. According to the results of scientific researches, it was found out that taxonomic studies, which were obtained by benefitting from morphological characteristic of Orthrias tigris, Orthrias angorae bureschi, Orthrias panthera and Cobitis taenia, were right.Öğe Feeding Habits of Tench (Tinca tinca L., 1758) in Beyşehir Lake (TURKEY)(Turkish Journal of Fisheries and Aquatic Sciences, 2010) Alaş, Ali; Altındağ, Ali; Yılmaz, Muhittin; Kırpık, M. Ali; Ak, AkifIn this study, alimentary canal contents of 188 tench (Tinca tinca L., 1758) caught from Beyşehir Lake (Turkey) between April 2004 and March 2005 were examined for feeding habits. Phytoplanktonic and zoooplanktonic organisms, insects, detritus (animal and vegetable) and two most common parasites of tench, Ligula intestinalis and Asymphylodora tincae were determined in observed in the alimentary canal of fish. There was a significant difference between the frequency of the existance of Chlorella, Coscinidiscus, Fragilaria, Mougetia, Phytoconis, Pinnularia, Rivularia, Synedra, Ulotrix, Brachionus, Gammarus sp., and Ligula intestinalis among seasons (df=3, P<0.05). Only 6 of these genera (Chaetophora, Coscinidiscus, Phytoconis, Surirella, Synedra, Daphnia) had a significant difference among age classes (df=5, P<0.05). Additionally, it was found that only 5 of the 188 tench (2.66%) were observed to have almost empty alimentary canal. Algae and macrophytes were found to be the most considerable food types in the present study, which is followed by zooplankton and insect larvae.Öğe Gnathia sp. (Gnathiidae) Infestations on Marine Fish Species from Turkey(Kafkas Univ. Veteriner Fakültesi Dergisi, 2009) Alaş, Ali; Öktener, Ahmet; Yılmaz, MuhittinThis study was carried out between June and August 2006. Totally 150 samples out of ten different fish species caught from the Marmara Sea, the Black Sea and the Aegean Sea were examined. For this purpose, body surface, fins, gills and mouth cavity of each fish were examined with the aid of a dissecting microscope. After that, ectoparasite samples were preserved in 70% ethanol to be observed under the dissecting microscope for identification. Gnathia sp. was found out in the mouth cavity and gill flaments of these infected fishes. This is the first study reporting Gnathia sp. from Serranus cabrilla; Mullus surmuletus; Mugil cephalus; Trachurus mediterraneus; Sarpa salpa; Sciaena umbra.Öğe Hekzavalent Kromun Capoeta capoeta (Guldenstaedt 1773) ve Squalius cephalus (Linnaeus 1758) Üzerine Olan Etkisinin Histopatolojik ve Elektroforetik Yöntemlerle Saptanması(Kafkas Univ. Veteriner Fakültesi Dergisi, 2013) Koç, Evren; Yılmaz, Muhitdin; Ersan, Yusuf; Alaş, AliÇalışmada, Kars Çayı’ndan yakalanan Siraz balığı (Capoeta capoeta) ve Tatlısu kefali (Squalius cephalus) üzerine hekzavalent kromun (CrVI) (potasyum dikromat olarak) etkileri histopatolojik ve elektroforetik yöntemlerle incelendi. Balıklar çeşme suyunda 10 gün süreyle bekletilerek ortama adaptasyonları sağlandı. Daha sonra, her bir balık türü için her grupta 10’ar adet olmak üzere 3 grup oluşturuldu. I. gruptaki (kontrol) balıklar çeşme suyu içeren tankta, II. gruptaki balıklar 10 gün süreyle, III. gruptaki balıklar ise 20 gün süreyle 10 mg/L dozunda CrVI içeren tanklarda bekletildi. Bu süre sonunda, balıklardan kan ve doku örnekleri alındı, daha sonra analizler yapıldı. Histopatolojik inceleme sonucunda CrVI’a maruz kalan Capoeta capoeta ve Squalius cephalus’ların karaciğer dokularında fokal nekroz alanları, hidropik ve vakuolar dejenerasyonlar tespit edildi. Bu dejenerasyonların şiddetinin hekzavalent kroma maruz kalma süresiyle artış gösterdiği gözlemlendi. Serum proteinlerinin SDS-PAGE’inde ise 10 gün süreyle CrVI uygulamasına bağlı olarak Capoeta capoeta’nın bazı protein bantlarında hafif kalınlaşma olduğu, Squalius cephalus’un protein bantlarında ise belirgin bir değişikliğin şekillenmediği saptandı. Hekzavalent kromun 20 gün süreyle uygulandığı Capoeta capoeta’nın birçok protein bantlarında incelmede, bazı protein bantlarında ise belirgin derecede kalınlaşmalar gözlendi. Squalius cephalus’da ise bazı protein bantlarında hafif derecede kalınlaşma meydana geldiği tespit edildi. Sonuç olarak; potasyum dikromat uygulamasının zamana bağlı olarak artan derecelerde Capoeta capoeta ve Squalius cephalus karaciğer dokularında ve protein ekspresyonlarında bozulmalara neden olduğu sonucuna varıldı.
