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Öğe Changes in Physiological Parameters and Some Antioxidant Enzymes Activities of Soybean (Glycine max L. Merr.) Leaves Under Cadmium and Salt Stress(Journal of Stress Physiology & Biochemistry, 2012) Aksoy, Merve; Seçkin Dinler, BurcuThe combined effect of cadmium stress (0,5 mM Cd(NO3)2) and salt stress (100 mM NaCl) on growth, lipid peroxidation and activities of some antioxidant enzymes were studied in soybean (Glycine max L. Merr.) leaves. Shoot lenghts were not changed under all groups. But fresh and dry weight were decreased under salt treatment alone and Cd treatment alone. The decrease was more in the group of combined of Cd and salt treatment. Although APX activity increased under salt treatment alone and combined of Cd and salt treatment, GR activity increased under Cd treatment alone and combined of Cd and salt treatment. Nevertheless DHAR activity increased only in combined of Cd and salt stress. MDA content increased under all groups but it was more in the combined of Cd and salt stress which indicates that salinity is more harmful with cadmium stress in soybean plants. Thus, it was concluded that some of antioxidant enzymes (APX, GR, DHAR) increased their activity under combined of Cd and salt treatment but they were not efficient to protect oxidative damage from soybean plants by alleviating the lipid peroxidation.Öğe DIFFERENT RESPONSES IN ACCLIMATED AND NON-ACCLIMATED PROCESSES OF SOYBEAN LEAVES TO SALINITY(Parlar Scientific Publications (P S P), 2014) Aksoy, Merve; Dinler, Burcu SeckinThe aim of the study is to determine different responses, occurring during the process of acclimation and non-acclimation in soybean (Glycine max L. Merr.) leaves which was not investigated before. With this aim, relative water content (RWC), relative growth rate (RGR), shoot length, lipid peroxidation and the activity of some antioxidant enzymes ((catalase (CAT, EC 1.11.1.6) ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.8.1.7)) were reported. In the experiment, the first group was exposed to an acclimation treatment (plants were watered with 100 mM NaCl from germination to leaf stage) and a second group was given a non-acclimated treatment (plants were watered with dIH(2)O from germination to leaf stage). After that, both groups were watered with 200 mM NaCl in a Hoagland solution for 7 days). The product of lipid peroxidation, MDA content, was increased in both groups but it was more pronounced in the non-acclimated group. The activities of CAT, APX and GR increased significantly in both groups, but this increase was higher in the acclimated group. As a result, acclimation process to salt stress was beneficial for soybean leaves by protecting them from oxidative damage with lower MDA content and higher enzyme activities of CAT, APX and GR.Öğe Drought tolerance of knotgrass (Polygonum maritimum L.) leaves under different drought treatments(Pakistan Journal of Botany, 2014) Seçkin Dinler, Burcu; Aksoy, MerveDrought stress is a major environmental stress factor for plants causing significant adverse effects on crop growth and yield. Therefore, plant resistance against drought should be determined for wild plants to alleviate such impacts. Knotgrass (Polygonum maritimum L.) is a wild plant getting rarer every day in sandy coasts. The present study was consulted to determine the responses of knotgrass (Polygonum maritimum L.) to drought stress during vegetative growth stages. Plants were drought-stressed by withholding water for (72 hours) and (10 days). Relative water content did not change under drought stress, but the chlorophyll content decreased in both drought treatments. Although, superoxide dismutase (SOD) and catalase (CAT) activity increased more in 10 days drought treatment than 72 hours treatment, ascorbate peroxidase (APX) and glutathione reductase (GR) activity of the treatments were not significantly different. Moreover, both malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents did not change under drought treatments. Such findings indicated Polygonum maritimum as a tolerant species to drought stress during vegetative growth stages.Öğe The Responses of Ascorbate - Glutathione Cycle Enzymes in Seedlings of Pancratium maritimum L. under Drought Treatments(Journal of Stress Physiology & Biochemistry, 2013) Seçkin Dinler, Burcu; Aksoy, MerveIn this study, physiological and biochemical responses of (Pancratium maritimum L.), desert plant which is very widespread on coastal sand dunes to drought were determined. Therefore 28 days (d) old plants were drought stressed by withholding water for 5 and 10 days. The changes in relative growth rate (RGR), relative water content (RWC) lipid peroxidation, and ascorbate-glutathione cycle enzymes activity ((ascorbate peroxidase (APX, EC 1.11.1.11), glutathione reductase (GR, EC 1.6.4.2) dehydroascorbate reductase (DHAR, EC 1.8.5.1) and monodehydroascorbate reductase (MDAR, EC 1.6.5.4)) were investigated. Relative growth rate, relative water content were both decreased on the 5 and 10d of stress treatment while it was higher on the 10d. MDA content increased on the 10d while it did not change on the 5d. On the other hand, activities of APX, GR, DHAR and MDAR increased on the 5d but were not change on the 10d. These results suggest that ascorbate – glutathione cycle enzymes were efficient to prevent from oxidative damage under short term of drought stress in (Pancratium maritimum L.) plants.