Sarsmaz, Hayrunnisa YesilGurgen, Seren GulsenSomdas, Mehmet Akif2025-03-232025-03-2320222147-2092https://doi.org/10.12996/gmj.2022.54https://hdl.handle.net/11486/5099Objective: To investigate of the effectiveness of N-acetylcysteine (NAC) in preventing cochlear damage due to gentamicin by the means of expression of neuronal factors Metods: In our study, 36 female Spraguea Dawley rats were used. They were divided into 3 groups such as (saline), Gentamicin + Serum physiological (saline) and Gentamicin + NAC. After 15 days, the rats were sacrificed. Cochleae were removed and examined histopathologically. The immunoreactivity of neurofilament and neuronal growth factor primary antibodies was examined in the tissues taken. Results: Neuroflament immunoreactivity was quite significant in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, whereas it was weak in the Gentamicin + serum physiological group. Also neuronal growth factor immunoreactivity was moderate in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, it was negative at vestibular ganglia, and weak in afferent and efferent nerve fiber bundles in the Gentamicin + serum physiological group. Conclusion: It was shown immunohistochemically that ototoxicity caused by gentamicin was significantly reduced when the NAC combination was applied.trinfo:eu-repo/semantics/openAccessGentamicinDrug Induced OtotoxicityN-acetylcysteineCochleaNerve growth factorNeurofilament proteinArticle33323223710.12996/gmj.2022.54Q4WOS:000823151400006N/A