Kurt, Ahmet ArifIbrahim, BasharCinar, HarunAtsu, Ayse NilhanBursalioglu, Ertugrul OsmanBayir, IsmailOzmen, Oezlem2026-04-252026-04-2520252310-2861https://doi.org/10.3390/gels11060431https://hdl.handle.net/11486/8590Hypericum perforatum L. (H.P.), a plant renowned for its wound-healing properties, was investigated for antioxidant/antimicrobial efficacy, toxicological safety, and in vivo wound-healing effects in this research to develop and characterize novel nanoemulsion hydrogel (NG) formulations. NG were prepared via emulsion diffusion-solvent evaporation and polymer hydration using Cremophor RH40 and Ultrez 21/30. A D-optimal design optimized oil/surfactant ratios, considering particle size, PDI, and drug loading. Antioxidant activity was tested via DPPH, ABTS+, and FRAP. Toxicological assessment followed HET-CAM (ICH-endorsed) and ICCVAM guidelines. The optimized NG-2 (NE-HPM-10 + U30 0.5%) demonstrated stable and pseudoplastic flow, with a particle size of 174.8 nm, PDI of 0.274, zeta potential of -23.3 mV, and 99.83% drug loading. Release followed the Korsmeyer-Peppas model. H.P. macerates/NEs showed potent antioxidant activity (DPPH IC50: 28.4 mu g/mL; FRAP: 1.8 mmol, Fe2+/g: 0.3703 +/- 0.041 mM TE/g). Antimicrobial effects against methicillin-resistant S. aureus (MIC: 12.5 mu g/mL) and E. coli (MIC: 25 mu g/mL) were significant. Stability studies showed no degradation. HET-CAM tests confirmed biocompatibility. Histopathology revealed accelerated re-epithelialization/collagen synthesis, with upregulated TGF-beta 1. The NG-2 formulation demonstrated robust antioxidant, antimicrobial, and wound-healing efficacy. Enhanced antibacterial activity and biocompatibility highlight its therapeutic potential. Clinical/pathological evaluations validated tissue regeneration without adverse effects, positioning H.P.-based nanoemulsions as promising for advanced wound care.eninfo:eu-repo/semantics/openAccesshydrogelnanoemulsionwound healingHET-CAMdrug delivery systemsantimicrobial activityHypericum perforatum L.in silico modelingCremophor RH40antioxidant activityArticle11610.3390/gels11060431405587302-s2.0-105009117562Q1WOS:001514997300001Q10000-0003-3086-09950000-0002-7273-3874