Corman, Mehmet Emin2025-03-232025-03-2320171303-50022687-475Xhttps://doi.org/10.15671/HJBC.2017.147https://search.trdizin.gov.tr/tr/yayin/detay/286567https://hdl.handle.net/11486/3779Extraction of trace level persistent organic pollutants is an important issue in water samples. So in this work, p(HEMAPA) monolithic columns were developed as a novel kind of solid-phase extraction adsorbents, as well as p(HEMAPA) solid-phase extraction (SPE) adsorbent was coupled with high-performance liquid chromatography (HPLC) (p(HEMAPA-HPLC) for the determination of benzo[a]pyrene (BaP) on-line mode. After p(HEMAPA-HPLC) parameters including the injection volume, flow rate, and the sample volume had been optimized in detail, BaP separation procedures on the p(HEMAPA-HPLC) containing mobile phase, different amounts of BaP, and reusability were performed. Advantages of this procedure are low quantitation limits (0.05–1.0 ng/mL), short extraction time, low consumption of organic solvents, and ease of automation and operation of the whole analysis. The experimental results indicated that p(HEMAPA-HPLC) allowed for one-step BaPs extraction, clean-up and analytical determination under the optimal conditions. The reusability of BaPs from water samples was 99.7% with a negligible capacity decrease after ten adsorption–desorption cycles. The method detection limit (MDL), relative standard deviation (RSD) and recovery were obtained as 0.007 ng/mL, 0.26%, and 97.0%, respectively. It was concluded that p(HEMAPA) monolithic column coupled with HPLC was an excellent alternative for the routine analysis of BaPs at trace level.eninfo:eu-repo/semantics/openAccessKimyaAnalitikKimyaUygulamalıÇevre BilimleriMühendislikKimyaArticle45214315110.15671/HJBC.2017.147286567