Polat, EceAltinbas, Mahmut2025-03-232025-03-2320231543-50751543-5083https://doi.org/10.1080/15435075.2022.2149269https://hdl.handle.net/11486/5720Mutagenesis in microbial strains might result in robust strains with enhanced biofuel properties. Besides that, oleaginous microalgae, Auxenochlorella sp. with enhanced biomass and lipid content, can be a strong candidate for biodiesel production. In this context, a chemical mutagen called ethyl methane sulfonate (EMS) was screened at different concentrations. Considering that a lower survival rate can result in a higher mutation rate, an EMS concentration of 0.25M with 13 mins exposure time was selected as the optimum mutation condition. An acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicide, tralkoxydim, was used as a selective environment to choose the mutant strains. Using an ACCase inhibitor herbicide would maintain a more desirable mutant strain that had the enhanced characteristics of biodiesel. Multi-scale comparisons between selected mutant strains and a wild strain revealed that by the time there was a remarkable decrease of 12.6% in the lipid content of the wild strain under the addition of Tralkoxydim (34.8%) compared to 39.8% in wild type, the lipid contents of the mutant strains remained higher. A remarkable increase by 1.50-fold and 1.33-fold in biodiesel fuel properties for Tralkoxydim-treated strains emphasizes that the ACCase synthesis pathway for lipid production is influenced by the stress factor that reaches the European biodiesel standards.eninfo:eu-repo/semantics/closedAccessBiodieselAuxenochlorella spAcetyl coenzyme a carboxylasefatty acid profileTralkoxydimArticle20111137114810.1080/15435075.2022.21492692-s2.0-85142633226Q2WOS:000889307100001Q2