Donmez, SonerArslan, FatmaSari, NursenOzkan, Elvan HasanogluArslan, Halit2025-03-232025-03-2320170885-45131470-8744https://doi.org/10.1002/bab.1533https://hdl.handle.net/11486/7320In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl) polystyrene (FMPS) with L-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 degrees C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 x 10(-4) to 1.0 x 10(-2) M, R-2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.eninfo:eu-repo/semantics/closedAccessbiosensorcarbon pasteglucose oxidasemicrospherepolystyreneSchiff baseArticle64574575310.1002/bab.1533276292722-s2.0-85018647138Q2WOS:000423086600015Q2