Armutcu, CananPiskin, SenaOzgur, ErdoganKarakaya, MustafaCorman, M. EminUzun, Lokman2026-04-252026-04-2520250026-36721436-5073https://doi.org/10.1007/s00604-025-07502-5https://hdl.handle.net/11486/8186A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 x 10-9 to 1 x 10-8 M, with a detection limit of 1.71 x 10-10 M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.eninfo:eu-repo/semantics/closedAccessHypoxanthineCNFsThymineMolecular imprintingModified glassy carbon electrodeDifferential pulse voltammetryArticle1921010.1007/s00604-025-07502-5409384322-s2.0-105015644260Q1WOS:001571593200002Q1